Plant thioesterases
AssigneeCalgene, Inc.
InventorsToni A. Voelker, Huw M. Davies
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Abstract
This invention relates to plant thioesterases, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant thioesterases. Also, by this invention, the existence of a heretofore unproven factor critical to the biosynthesis of medium-chain fatty acids in plants is demonstrated.
Claims16
A recombinant DNA construct comprising a plant C10 or C12 preferring thioesterase encoding sequence, wherein said sequence comprises the thioesterase encoding sequence of FIG. 6, and wherein the initiation codon for translation of said thioesterase encoding sequence is the ATG codon of positions 145-147 of the sequence shown in FIG. 6.
A recombinant DNA construct comprising a plant C10 or C12 preferring acyl-ACP thioesterase encoding sequence.
A recombinant DNA construct comprising as operably linked components in the 5' to 3' direction of transcription, a transcriptional initiation region functional in a host plant or bacterial cell and a plant C10 or C12 preferring acyl-ACP thioesterase nucleic acid sequence, wherein said transcriptional initiation region is not naturally linked to said thioesterase sequence.
A DNA construct comprising an expression cassette capable of producing a plant thioesterase in a plant cell comprising as operably linked components in the 5' to 3' direction of transcription, a transcriptional initiation regulatory region functional in said plant cell, a translational initiation regulatory region functional in said plant cell, a sense DNA sequence encoding a biologically active plant C10 or C12 preferring acyl-ACP thioesterase, and a transcriptional termination regulatory region functional in said plant cell, wherein at least one of said regulatory regions is not naturally linked to said sense DNA sequence.
A recombinant DNA construct comprising a plant thioesterase encoding sequence, wherein said sequence comprises a nucleotide sequence encoding the amino acid sequence IRxYEVG from position 105 to 111 of SEQ ID NO:
- 45, and from position 113 to 119 of SEQ ID NO:
- 42.
A recombinant DNA construct comprising a plant thioesterase encoding sequence, wherein said sequence comprises a nucleotide sequence encoding the amino acid sequence NxHVNN from position 279 to 284 of SEQ ID NO:
- 45, and from position 283 to 288 of SEQ ID NO:
- 42.
A recombinant DNA construct comprising a plant thioesterase encoding sequence, wherein said sequence comprises a nucleotide sequence encoding the amino acid sequence TLxYRREC, from position 309 to 316 of SEQ ID NO:
- 45, and position 313 to 320 of SEQ ID NO:
- 42.
Dependencies are inferred from the claim text.