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US 5,512,479 AGranted
Method of degrading volatile organochlorides and remediation thereof
AssigneeEnvirogen, Inc.
InventorRobert J. Steffan
Life of this patent
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Abstract
A process for degrading volatile organic contaminants such as trichloroethylene. and remediating volatile organic contaminant contaminated media using trichloroethylene induced toluene monooxygenase activity in Pseudomonas mendocina KR-1 is described.
Claims21
A method of degrading trichloroethylene in a medium contaminated with a 1 .mu.M or higher concentration of said trichloroethylene, said method comprising the steps of:
- (a) introducing a degradation effective mount of Pseudomonas mendocina KR-1 ATCC 55706 into said medium under conditions sufficient to degrade said trichloroethylene;
- (b) inducing, with said trichloroethylene, toluene monooxygenase genes naturally occurring within said Pseudomonas mendocina KR-1 ATCC 55706;
- and
- (c) degrading with said Pseudomonas mendocina KR-1 ATCC 55706 and with said toluene monooxygenase genes induced by said trichloroethylene at least a portion of said trichloroethylene in said medium.
1 more element
A method of degrading a volatile organochloride comprising:
- contacting degradation effective amounts of Pseudomonas mendocina KR-1 ATCC 55706 and trichloroethylene with said volatile organochloride;
- and
- degrading with said Pseudomonas mendocina KR-1 ATCC 55706 said volatile organochloride.
A method of degrading trichlorethylene comprising:
- contacting trichloroethylene with a degradation effective mount of Pseudomonas mendocina KR-1 ATCC 55706;
- and
- catalyzing a degradation of said trichloroethylene by induction of toluene monooxygenase genes naturally occurring within said Pseudomonas mendocina KR-1 ATCC 55706 with said trichloroethylene.
A method of remediating a medium contaminated with at least one volatile organochloride comprising:
- contacting said at least one volatile organochloride with a degradation effective amount of Pseudomonas mendocina KR-1 ATCC 55706;
- and
- degrading with said Pseudomonas mendocina KR-1 ATCC 55706 said at least one volatile organochloride by contacting said Pseudomonas mendocina KR-1 with a degradation effective mount of trichloroethylene.
Dependencies are inferred from the claim text.