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US 5,512,475 AGranted
Three-dimensional skin cell and tissue culture system
AssigneeAdvanced Tissue Sciences, Inc.
InventorsGail K. Naughton, Brian A. Naughton
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AssigneeAdvanced Tissue Sciences, Inc.
InventorsGail K. Naughton, Brian A. Naughton
Estimating the term…
The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.
A method for transplantation or implantation of a three-dimensional skin cell culture comprising implanting a culture of melanocyte and keratinocyte cells cultured on a living stromal tissue prepared in vitro, said living stromal tissue comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a framework composed of a biocompatible, non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells so that a tissue equivalent is formed.
A method for transplantation or implantation of a three-dimensional culture comprising implanting in vivo melanocytes and keratinocytes cultured on a living stromal tissue prepared in vitro, said living stromal tissue comprising confluent stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a framework composed of a biocompatible, non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells so that a tissue equivalent is formed.
A method for determining the effect of a drug on melanocyte and keratinocyte cells in culture comprising:
A method for studying the mechanisms of a disease or condition in a patient, which disease or condition has a detectable affect on melanocyte and keratinocyte cells in culture, comprising:
Dependencies are inferred from the claim text.