Process for purifying an .alpha.-D-galactosidase isozyme from Coffea beans
AssigneeThe Curators of the University of Missouri
InventorDaniel S. Smith
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Abstract
A Coffea canephora-D-galactosidase isozyme is purified by extracting a supernatant from Coffea beans containing the isozyme, extracting tannin from the supernatant, and isolating the isozyme. Preferrably, the supernatant is extracted by exposing the supernatant to insoluble polyvinylpolypyrrolidone in an amount sufficient to remove the tannin from the supernatant by forming hydrogen bonds with the tannin.
Claims19
A process for purifying a Coffea canephora .alpha.-D-galactosidase isozyme by 1) homogenizing coffee beans containing the isozyme with an extraction buffer and obtaining a supernatant containing the isozyme and tannin, 2) extracting tannin from the supernatant by exposing the supernatant to insoluble polyvinylpolypyrrolidone (PVPP) in an amount sufficient to remove tannin from the supernatant by forming hydrogen bonds with the tannin, and 3) isolating the isozyme from the supernatant from which tannin has been removed.
Dependencies are inferred from the claim text.