Enzymatic acylation of 3-hydroxymethyl cephalosporins
AssigneeBristol-Myers Squibb Company
InventorsJohn J. Usher, Guna Romancik, Michael Politino, David A. Lowe
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Abstract
O-acylated cephalosporins are produced by reacting 3-hydroxymethylcephalosporins with an acyl donor containing at least three carbon atoms and an enzyme which is a Rodosporidium toruloides esterase, a wheat germ lipase, an Aspergillus niger lipase, an orange peel acetylesterase or a Bacillus subtilis esterase. A preferred enzyme is the esterase from Rodosporidium toruloides ATCC 10657. The enzyme can be used while in whole cells or in soluble or inmobilized form.
Claims20
An acylation process for the preparation of a cephalosporin compound of the formula ##STR40## wherein R' is hydrogen or an acyl group of the formula ##STR41## wherein R is the residue of a carboxylic acid, X is hydrogen or halo, and
- G is sulphur, oxygen, sulphoxide, sulphone, or methylene;
- which comprises reacting in an aqueous medium a compound of the formula ##STR42## with an acyl donor containing at least three carbon atoms and an enzyme selected from the group consisting of Rodosporidium toruloides esterase, wheat germ lipase, Aspergillus niger lipase, orange peel acetylesterase and Bacillus subtilis esterase whereby said cephalosporin compound is produced.
Dependencies are inferred from the claim text.