Test device for determining the presence of leukocyte cells, esterase or protease in a test sample
AssigneeBayer Aktiengesellschaft
InventorsGary M. Johnson, Robert J. Schaeper
Life of this patent
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Abstract
A test device for determining the presence of leukocyte cells, esterase or protease in a test sample is disclosed. The test device comprises a carrier matrix having a reagent composition incorporated therein, wherein the reagent composition comprises a lactate ester having the structure ##STR1## wherein A is an alcohol blocking group, and wherein B--O-- is a residue of compound B--OH and provides a detectable response when the lactate ester is hydrolyzed; and a buffer. In a preferred embodiment, the lactate ester has the structure ##STR2## wherein X is O, S or NR.sup.2, R is aryl or lower alkyl, R.sup.1 is hydrogen or lower alkyl, and R.sup.2 is hydrogen, lower alkyl or aryl.
Claims8
A test device for determining the presence or concentration of leukocyte cells, esterase or protease in a test sample, said test device comprising a test pad, wherein the test pad comprises a carrier matrix having a reagent composition homogeneously incorporated therein, said reagent composition comprising:
- (a) a lactate ester having the structure ##STR18## wherein A is an alcohol blocking group, and wherein B--O-- is a residue of a compound B--OH and provides a detectable response when the lactate ester is hydrolyzed to generate the compound B--OH;
- and
- (b) a buffer.
An analyte detection device to determine the presence or concentration of human leukocyte elastase or leukocyte cells in a liquid test sample comprising:
- a support strip;
- a reagent test pad attached to the support strip;
- and
- a reagent composition incorporated into the reagent test pad, said reagent composition comprising
- (a) a lactate ester having the structure ##STR21## wherein A is an alcohol blocking group, and wherein B--O-- is a residue of a compound B--OH and provides a detectable response when the lactate ester is hydrolyzed to generate the compound B--OH;
- (b) a buffer,
- (c) an accelerator compound which increases the rate of hydrolysis of the lactate ester;
- and
- (d) a diazonium salt.
Dependencies are inferred from the claim text.