Method for the functional determination of protein C inhibitor
AssigneeBehringwerke Aktiengesellschaft
InventorsThomas Stief, Norbert Heimburger, Klaus-Peter Radtke
Life of this patent
Estimating the term…
Abstract
A method and an agent for the functional determination of protein C inhibitor in body fluids are described, in which this fluid is incubated with a known amount of a plasminogen activator, fibrinogen and a sulfated carbohydrate, and the residual activity of the plasminogen activator is determined, and from this the amount of protein C inhibitor is determined.
Claims9
A method for the functional determination of protein C inhibitor in a body fluid, which method comprises the steps of:
- (a) incubating a sample of said fluid with a urokinase comprising a mixture of a low-molecular-mass (LMM) form of about 33 kD molecular weight and a high-molecular-mass (HMM) form of about 54 kD molecular weight, and having a potency of not less than 70,000 IU/mg, in a concentration of 0.1-5,000 IU/ml, in the presence of a sulfated carbohydrate in a concentration of 10.sup.-8 to 1 mg/ml, the amounts of said urokinase and said sulfated carbohydrate being sufficient to give a readable signal of activity of said urokinase after inhibition by said protein C inhibitor, (b) determining the residual activity of said urokinase, and (c) from this residual activity of said urokinase, determining the amount of protein C inhibitor in said fluid by comparing said residual activity of said urokinase with that activity obtained with a standard solution of known concentration of protein C inhibitor.
A method for the functional determination of protein C inhibitor in a body fluid, which method comprises the steps of:
- (a) incubating a sample of said fluid with a urokinase comprising a mixture of a low-molecular-mass (LMM) form of about 33 kD molecular weight and of a high-molecular-mass (HMM) form of about 54 kD molecular weight, and having a potency of not less than 70,000 IU/mg, in a concentration of 0.1-5,000 IU/ml in the presence of a sulfated carbohydrate in a concentration of 10.sup.-8 to 1 mg/ml and of fibrinogen, the amounts of said urokinase and said sulfated carbohydrate being sufficient to give a readable signal of activity of said urokinase after inhibition by said protein C inhibitor, (b) measuring the residual activity of said urokinase, and (c) determining the amount of protein C inhibitor in said fluid by comparing said residual activity of said urokinase with that activity obtained with a standard solution of known concentration of protein C inhibitor.
A reagent for determining protein C inhibitor comprising 0.1-5,000 IU/ml urokinase, comprising a mixture of a low-molecular-mass (LMM) form of about 33 kD molecular weight and a high-molecular-mass (HMM) form of about 54 Kd molecular weight, and having a potency of not less than 70,000 IU/mg 10.sup.-7 -10.sup.-1 g/l a sulfated carbohydrate, 5.times.10.sup.-5 to 5 mg/ml fibrinogen, and a nonionic detergent in a buffer solution of pH 6-9.
Dependencies are inferred from the claim text.