Methods for the detection of Chlamydia trachomatis

A method of detecting the presence of Chlamydia trachomatis nucleic acids in a sample comprising:

• a) contacting said sample with a first oligonucleotide which hybridizes to rRNA or rDNA of Chlamydia trachomatis and not to rRNA or rDNA of non-Chlamydia bacteria under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes wherein said oligonucleotide is complementary to a region of Chlamydia trachomatis rRNA or a region of DNA encoding said rRNA and has a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:46, and
• ii) RNA equivalents thereof having uracil substitutes for thymine,
• and conservatively modified variants thereof, and a second oligonucleotide which hybridizes to rRNA or rDNA of Chlamydia trachomatis under said conditions and has a nucleotide sequence selected from the group consisting of:
• iii) SEQ ID NO:48, and
• iv) RNA equivalents thereof having uracil substitutes for thymine,
• and conservatively modified versions thereof,
• b) imposing said hybridization conditions on said sample and said first and second oligonucleotides to allow the oligonucleotides to hybridize to nucleic acids encoding Chlamydia trachomatis rRNA or rDNA sequencers, if present, to form a stable hybrid, and
• c) detecting said hybrid, if present in said sample, as an indication of the presence of Chlamydia trachomatis nucleic acids in the sample.

A method for the detection of Chlamydia trachomatis comprising:

• a) amplifying Chlamydia trachomatis nucleic acids with at least one amplification oligonucleotide that will bind to or cause polymerization through a region of Chlamydia trachomatis nucleic acids of between 22 and 100 nucleotides, said region having a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:
• 12,
• ii) SEQ ID NO:
• 5,
• iii) SEQ ID NO:
• 6,
• iv) SEQ ID NO:
• 11,
• v) RNA equivalents of these sequences having uracil substituted for thymine, and
• b) detecting the amplified nucleic acids with an oligonucleotide hybridization assay probe which will specifically hybridize with Chlamydia trachomatis nucleic acids over nucleic acids of Chlamydia psittaci and Chlamydia pneumoniae under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes.

A method for the detection of Chlamydia trachomatis comprising:

• a) amplifying Chlamydia trachomatis nucleic acids with at least one amplification oligonucleotide that will bind to or cause polymerization through a region of Chlamydia trachomatis nucleic acids of between 22-100 nucleotides, said region having a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:
• 2,
• ii) SEQ ID NO:
• 4,
• iii) SEQ ID NO:
• 13,
• iv) SEQ ID NO:
• 14, and
• v) RNA equivalents of these sequences having uracil substituted for thymine, and
• b) detecting the amplified nucleic acids with an oligonucleotide hybridization assay probe which will specifically hybridize with Chlamydia trachomatis nucleic acids over nucleic acids of Chlamydia psittaci and Chlamydia pneumoniae under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes.

A method for the detection of Chlamydia trachomatis comprising:

• a) amplifying Chlamydia trachomatis nucleic acids with at least one amplification oligonucleotide that will bind to or cause polymerization through a region of Chlamydia trachomatis nucleic acids of between 24-100 nucleotides, said region having a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:
• 3,
• ii) SEQ ID NO:
• 8,
• iii) SEQ ID NO:
• 15, and
• iv) RNA equivalents of these sequences having uracil substituted for thymine, and
• b) detecting the amplified nucleic acids with an oligonucleotide hybridization assay probe which will specifically hybridize with Chlamydia trachomatis nucleic acids over nucleic acids of Chlamydia psittaci and Chlamydia pneumoniae under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes.

A method for the detection of Chlamydia trachomatis comprising:

• a) amplifying Chlamydia trachomatis nucleic acids with at least one amplification oligonucleotide that will bind to or cause polymerization through a region of Chlamydia trachomatis nucleic acids of between 24-100 nucleotides, said region having a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:
• 10,
• ii) SEQ ID NO:
• 7,
• iii) SEQ ID NO:
• 16, and
• iv) RNA equivalents thereof having uracil substituted for thymine, and
• b) detecting the amplified nucleic acids with an oligonucleotide hybridization assay probe which will specifically hybridize with Chlamydia trachomatis nucleic acids over nucleic acids of Chlamydia psittaci and Chlamydia pneumoniae under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes.

A method for the detection of Chlamydia trachomatis comprising:

• a) amplifying Chlamydia trachomatis nucleic acids with at least one amplification oligonucleotide that will bind to or cause polymerization through a region of Chlamydia trachomatis nucleic acids of between 27-100 nucleotides, said region having a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:
• 44,
• ii ) SEQ ID NO:
• 51,
• iii) SEQ ID NO:
• 48, and
• iv) RNA equivalents of these sequences having uracil substituted for thymine, and
• b) detecting the amplified nucleic acids with an oligonucleotide hybridization assay probe which will specifically hybridize with Chlamydia trachomatis nucleic acids over nucleic acids of Chlamydia psittaci and Chlamydia pneumoniae under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes.

A method for the detection of Chlamydia trachomatis comprising:

• a) amplifying Chlamydia trachomatis nucleic acids with at least one amplification oligonucleotide that will bind to or cause polymerization through a region of Chlamydia trachomatis nucleic acids of between 27-100 nucleotides, said region having a nucleotide sequence selected from the group consisting of:
• i) SEQ ID NO:
• 49,
• ii) SEQ ID NO:
• 45,
• iii) SEQ ID NO:
• 50, and
• iv) RNA equivalents of these sequences having uracil substituted for thymine, and
• b) detecting the amplified nucleic acids with an oligonucleotide hybridization assay probe which will specifically hybridize with Chlamydia trachomatis nucleic acids over nucleic acids of Chlamydia psittaci and Chlamydia pneumoniae under hybridization conditions corresponding to 0.8M monovalent cation at 60.degree. C. for 15 minutes.