Method detecting antigens and/or antibodies
AssigneeStiftung fur Diagnostische Forschung
InventorsYves Lapierre, Dieter Josef, Jean Adam, Susanne Greber-Widmer
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Abstract
A suspension of inert particles is prepared in an aqueous solution, to which an antibody or an antigen and a carrier-bound antigen or antibody, respectively, are added in any desired order. After centrifuging, the positive, weakly positive, or negative reaction can easily be recognized on the basis of a simple pattern.
Claims2
A method of detecting target antibodies or antigens by reaction with specific binding partners thereto, wherein one but not both of the target or the binding partner is bound to a carrier, wherein a complex of the carrier-bound target and the binding partner, or of the target and the carrier-bound binding partner, forms an agglutinate which is optically detectable, said method comprising:
- providing a microreaction vessel containing a slurry or suspension of inert particles and the known target or binding partner thereto;
- adding to the vessel about 10-150 .mu.l of a solution containing the carrier-bound target or binding partner to allow formation of a target-binding partner complex which is carrier-bound and optically detectable;
- centrifuging the vessel;
- and
- observing the location of the carrier to determine the presence of the target antibody or antigen to be detected, with a strongly positive reaction being indicated by the carrier lying upon or in a top portion of a layer of the inert particles, and a negative reaction being indicated by the carrier-bound target or carrier-bound binding partner lying beneath the inert particles.
A method of detecting analyte antibodies or antigens in a fluid sample, by reaction with known antigen or antibody specific binding partners thereto, respectively, wherein one, but not both, of the analyte or known antigen or antibody is bound to a carrier, said method comprising:
- providing a microreaction vessel containing a slurry or suspension of inert particles and anti-immunoglobin;
- adding to the reaction vessel a fluid sample of about 10-150 .mu.l which may contain the analyte;
- adding to the vessel about 10-150 .mu.l of a solution containing the carrier-bound antigen or antibody to allow formation of an optically detectable complex between a) the anti-immunoglobin, and b) the carrier-bound antigen and the analyte antibody, or the carrier-bound antibody and the analyte antigen;
- centrifuging the vessel;
- and
- observing the location of the complex to determine the presence of the analyte antibody or antigen, with a strongly positive reaction being indicated by the complex lying upon or in a top portion of a layer of the inert particles, and a negative reaction being indicated by the absence of a complex, with the carrier-bound antigen or antibody lying beneath the inert particles.
Dependencies are inferred from the claim text.