Process of making resuspendable coated magnetic particles
AssigneeImmunivest Corporation
InventorsPaul A. Liberti, Steven P. Piccoli
Life of this patent
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Abstract
A method is provided for direct coating of transiently stable, colloidal particulate magnetic substrates with various synthetic or naturally-occurring polymers, particularly biologically-active peptides and proteins. The resulting coated resuspendable, colloidal magnetic particles are particularly adapted for use in bioanalytical procedures involving affinity separation, or the like. There is also provided a method for selectively binding chemically-treated colloidal magnetic particles to polyelectrolytes, e.g., to facilitate extraction of nucleic acids from cell lysates.
Claims22
A process for making resuspendable, coated magnetic particles by direct application of a coating material to a particulate magnetic substrate, said process comprising:
- a. dividing at least one particle of a magnetic starting material into a plurality of aggregable, smaller sized particles, thereby to provide an uncoated particulate magnetic substrate;
- b. forming a suspension of said uncoated particulate magnetic substance in a liquid medium;
- and
- c. contacting said uncoated, suspended particulate magnetic substance with said coating material, before substantial particulate magnetic substrate aggregation occurs, for a time sufficient for said coating material to adhere to said substrate, thereby forming said resuspendable, coated magnetic particles.
A process for making a stable suspension of resuspendable magnetic particles, said process comprising:
- a. forming a liquid mixture of a particulate magnetic starting material;
- b. sonicating said mixture to divide said particulate magnetic starting material into a plurality of uncoated aggregable, smaller sized particles suspended in a liquid;
- c. contacting said uncoated, suspended smaller sized particles with said coating material, before substantial aggregation of said smaller sized particle occurs, for a time sufficient for said coating material to adhere to said substrate, thereby forming said resuspendable, coated magnetic particles.
- d. recovering said resuspendable, coated magnetic particle.
Dependencies are inferred from the claim text.