Method of preparing a thromboplastin extract
AssigneeHelena Laboratories Corporation
InventorsDavid A. Barrow, Richard L. Rullman
Life of this patent
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Abstract
A thromboplastin extract including an aqueous thromboplastin extract of a powdered thromboplastin source including a metal ion chelator. A thromboplastin reagent for use in blood coagulation tests, the thromboplastin reagent includes the extract and calcium ions, and may include one or more of a stabilizer and a preservative. A process for preparing a thromboplastin extract including extracting a powdered thromboplastin source in an aqueous solution having a metal ion chelator, and separating the powder in solution into sedimented powder and supernatant thromboplastin extract. The supernatant thromboplastin extract is mixed with calcium ions, and may be mixed with one or more of a stabilizer and a preservative, to prepare thromboplastin reagent.
Claims12
A process for preparing a thromboplastin reagent comprising:
- providing an aqueous thromboplastin extract of a powdered thromboplastin source and a metal ion chelator;
- and
- mixing said thromboplastin extract with a source of calcium ions to provide a thromboplastin reagent.
A process for preparing a thromboplastin extract comprising:
- providing a powdered thromboplastin source comprising powdered human placenta;
- extracting said powdered thromboplastin source in an aqueous solution comprising a metal ion chelator;
- separating said powdered thromboplastin source in aqueous solution into sedimented powder and supernatant thromboplastin extract;
- and
- isolating said supernatant thromboplastin extract.
Dependencies are inferred from the claim text.