Cloning of the recA gene from thermus aquaticus YT-1
AssigneeThe United States of American as represented by the Secretary of Health and Human Services
InventorsRafael D. Camerini-Otero, Evelina Angov
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Abstract
The present invention includes the isolation of an oligonucleotide encoding Thermus aquaticus recA protein, and to the purified protein. The invention also includes methods of use of the protein, particularly, methods for hybridizing a primer to a complementary template with increased binding affinity at a temperature above 45.degree. C.
Claims6
An isolated DNA molecule encoding Thermus Aquaticus recA protein as shown in SECt ID NO:2.
An isolated DNA molecule, wherein said DNA molecule has a sequence complementary to the nucleotide sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.