Scavenger receptor protein and antibody thereto
AssigneeMassachusetts Institute of Technology
InventorsMonty Krieger, Tatsuhiko Kodama
Life of this patent
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Abstract
A substantially pure receptor protein capable of binding acetylated low density lipoprotein and oxidized low density lipoprotein is disclosed herein. This protein is characterized by having a molecular weight of about 220,000 daltons, and an affinity for oxidized low density lipoprotein and acetylated low density lipoprotein. Further, it is an integral membrane protein which includes a collagen domain. Proteins having an affinity for the receptor protein as well as DNA sequences encoding at least a portion of the receptor protein are also disclosed herein. In addition, devices for purification purposes and methods for detecting atherosclerotic plaques are described, both of which utilize the receptor protein or binding proteins thereto.
Claims17
A substantially pure receptor protein capable of binding acetylated low density lipoprotein, said protein found on the surface of macrophages and characterized by:
- being formed by combining three isolated subunits, each having an apparent molecular weight on polyacrylamide gels of about 77,000 daltons, the protein when glycosylated having an apparent molecular weight on SDS-polyacrylamide gels of about 220,000 daltons, and having a capacity for acetylated low density lipoprotein of approximately 1.4 mg of Acetyl-LDL protein/mg receptor protein, wherein the subunits are encoded by a DNA sequence specifically hybridizing to the DNA sequence shown in FIGS. 3A, 3B and 3C or FIGS. 4A, 4B and 4C.
An isolated 77,000 dalton protein subunit of a receptor protein capable of binding acetylated low density lipoprotein, wherein three of the isolated subunits, each having an apparent molecular weight on polyacrylamide gels of about 77,000 daltons, form a protein on the surface of macrophages, which when glycosylated has an apparent molecular weight on SDS-polyacrylamide gels of about 220,000 daltons.
An isolated DNA molecule encoding a substantially pure receptor protein capable of binding acetylated low density lipoprotein, said protein found on the surface of macrophages and formed by combining three isolated subunits, each having an apparent molecular weight on polyacrylamide gels of about 77,000 daltons, the protein when glycosylated having an apparent molecular weight on SDS-polyacrylamide gels of about 220,000 daltons.
A substantially pure receptor protein, wherein the protein is capable of binding acetylated low density lipoprotein when the protein is found on the surface of macrophages, and wherein the protein is characterized by being formed by combining three isolated subunits, each having an apparent molecular weight on polyacrylamide gels of about 77,000 daltons, the protein when glycosylated having an apparent molecular weight on SDS-polyacrylamide gels of about 220,000 daltons, and having a capacity for acetylated low density lipoprotein of approximately 1.4 mg of Acetyl-LDL protein/mg receptor protein, having at least one subunit not including the transmembrane and cytoplasmic region.
Dependencies are inferred from the claim text.