IL-1.beta.protein precursor
AssigneesNew England Medical Centers Hospitals, Inc., Trustees of Tufts College, Wellesley College, Massachusetts Institute of Technology
InventorsPhilip E. Auron, Charles A. Dinarello, Andrew C. Webb, Alexander Rich, Sheldon M. Wolff
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Abstract
The subject invention concerns a nucleic acid comprising a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Specifically, the subject invention comprises the cloning of a cDNA synthesized by reverse transcription of poly(A)RNA isolated from adherent human monocytes-stimulated with bacterial endotoxin. Human IL-1 is useful to induce the production of IL-2 by activated T-cells; it also acts on B-cells and NK-cells.
Claims1
A substantially pure protein having the following amino acid sequence:
- M A E V P K L A S E M M A Y Y S G N E D D L F F E A.sub.26 D G P K Q M K G S F Q D L D L C P L D G G I Q L R I.sub.52 S D H H Y S K G F R Q A K S V V V A M D K L R K M L.sub.78 V P C P Q T F Q E N D L S T F F P F I F E E E P I F.sub.104 F D T W D N E A Y V H D A P V R S L N C T L R D S Q.sub.130 Q K S L V M S G P Y E L K A L H L Q G Q D M E Q Q V.sub.156 V F S M S F V Q G E E S N D K I P V A L G L K E K N.sub.182 L Y L S C V L K D D K P T L Q L E S V D P K N Y P K.sub.208 K K M E K R F V F N K I E I N N K L E F E S A Q F P.sub.234 N W Y I S T S Q A E N M P V F L G G T K G G Q D I T.sub.260 D F T M Q F V S S.sub.269.
Dependencies are inferred from the claim text.