Growth of pancreatic islet-like cell clusters
AssigneeDesmos, Inc.
InventorsVito Quaranta, Jonathan C. R. Jones
Life of this patent
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Abstract
A method for growing endocrine precursor cells in vitro, such as pancreatic islet precursors, by culturing such cells in the presence or absence of cell matrix proteins capable of promoting hemidesmosome formation, such as those produced by the rat bladder carcinoma cell line 804G.
Claims7
A method for growing cells of pancreatic islet-like cell clusters (ICCs), comprising the step of culturing said ICCs in contact with a laminin-like extracellular matrix material that is produced by 804G cells and NBT-II cells, wherein said matrix material comprises three polypeptides having molecular weights of about 150 kD, 140 kD and 135 kD, said matrix material characterized as:
- (a) promoting enhanced growth of said ICCs in comparison to ICCs grown without the extracellular matrix material;
- (b) having the ability to promote hemidesmosome formation in epithelial cells cultured thereon;
- (c) binding concanavalin;
- and
- (d) being bound by polyclonal antibodies generated against the extracellular matrix secreted by 804G or NBT II cells.
Dependencies are inferred from the claim text.