Method of controlling the degradation of glycoprotein oligosaccharides produced by cultured Chinese hamster ovary cells
AssigneeThe Board of Trustees of the Leland Stanford Juniot University
InventorsCharles F. Goochee, Michael J. Gramer
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Abstract
A neuraminidase enzyme with unusually high activity under extracellular culture conditions has been discovered in Chinese hamster ovary (CHO) cells and other types of cells. By affecting the neuraminidase activity of the cell culture, the degradation of glycoprotein oligosaccharides produced by these cultured cells can be controlled.
Claims13
An in vitro method of controlling the degradation of glycoprotein oligosaccharides produced by cultured Chinese hamster ovary (CHO) cells, said method comprises:
- culturing CHO cells in a culture medium under conditions selected to increase or decrease the culture's extracellular neuraminidase activity;
- thereby maintaining or decreasing the sialic acid content of said glycoprotein oligosaccharides.
An in vitro method of controlling the degradation of glycoprotein oligosaccharides produced by cultured Chinese hamster ovary (CHO cells, said method comprises:
- (a) culturing CHO cells in a culture medium;
- and
- (b) decreasing the culture's extracellular neuraminidase activity, thereby maintaining the sialic acid content of said glycoprotein oligosaccharides.
Dependencies are inferred from the claim text.