Plants resistant to infection by PLRV
AssigneeMonsanto Company
InventorsTimothy A. Mitsky, Cynthia L. Hemenway, Nilgun E. Tumer
Life of this patent
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Abstract
An isolated DNA sequence which codes for a PLRV replicase gene is disclosed herein. A method for providing resistance to infection by a virus by expressing a replicase gene in plants is also disclosed, as are transgenic potato plants and tubers containing the replicase gene.
Claims21
A DNA molecule which comprises:
- (a) a promoter region which functions in plant cells to cause the production of an RNA sequence;
- which is operably linked to
- (b) a structural gene encoding a full length potato leafroll virus replicase;
- which is operably linked to
- (c) a 3' non-translated region which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the transcribed mRNA sequence.
A method for providing resistance to infection by potato leafroll virus in a susceptible Solanaceae plant which comprises:
- (a) transforming plant cells with a DNA molecule which comprises:
- (i) a promoter region which functions in plant cells to cause the production of an RNA sequence;
- which is operably linked to
- (ii) a structural gene encoding a full length potato leafroll virus replicase;
- which is operably linked to
- (iii) a 3' non-translated region which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the transcribed mRNA sequence;
- (b) regenerating said plant cells to provide a differentiated plant;
- and
- (c) selecting a transformed plant which expresses the full length potato leafroll virus replicase gene at a level sufficient to render the plant resistant to infection by said potato leafroll virus.
A method for providing reduction in net necrosis resulting from infection by potato leafroll virus in a susceptible Solanaceae plant which comprises:
- (a) transforming plant cells with a DNA molecule which comprises:
- (i) a promoter region which functions in plant cells to cause the production of an RNA sequence;
- which is operably linked to
- (ii) a structural gene encoding a full length potato leafroll virus replicase;
- which is operably linked to
- (iii) a 3' non-translated region which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the transcribed mRNA sequence;
- (b) regenerating said plant cells to provide a differentiated plant;
- and
- (c) selecting a transformed plant which expresses the full length potato leafroll virus replicase gene at a level sufficient to render the plant resistant to infection by said potato leafroll virus.
A virus resistant transformed Solanaceae plant which contains in its genome a DNA molecule which comprises:
- (a) a promoter region which functions in plant cells to cause the production of an RNA sequence;
- which is operably linked to
- (b) a structural gene encoding a full length potato leafroll virus replicase;
- which is operably linked to
- (c) a 3' non-translated region which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the transcribed mRNA sequence.
Dependencies are inferred from the claim text.