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US 5,510,245 AGranted
Composition and method of assaying for ketone bodies
AssigneeBayer Aktiengesellschaft
InventorThomas A. Magers
Life of this patent
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AssigneeBayer Aktiengesellschaft
InventorThomas A. Magers
Estimating the term…
A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-.beta.-hydroxybutyrate, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with D-.beta.-hydroxybutyrate to produce a detectable or measurable response. In addition, a new and improved indicator reagent composition, comprising a) an indicator dye that is responsive to thiols, such as a substituted isobenzothiazolone, Ellman's reagent or a derivative of Ellman's reagent; b) D-.beta.-hydroxybutyrate dehydrogenase; c) lipoamide dehydrogenase; d) D,L-lipoamide; and e) nicotinamide adenine dinucleotide, is incorporated into the carrier matrix to provide an accurate and sensitive assay of a test sample for D-.beta.-hydroxybutyrate (DHBA) in particular, and for ketone bodies in general. The improved method and composition are especially useful in the assay of whole blood, blood serum, blood plasma and urine for ketone bodies.
A method of determining the presence or concentration of D-.beta.-hydroxybutyrate in a test sample consisting essentially of:
A method of determining the presence or concentration of d-.beta.-hydroxybutyrate in a test sample consisting essentially of:
A method of determining the concentration of total ketone bodies in a biological fluid consisting essentially of:
A method of determining the presence or concentration of D-.beta.-hydroxybutyrate in a liquid sample consisting essentially of:
An analyte detection device to determine the presence or concentration of D-.beta.-hydroxybutyrate in a liquid test sample consisting essentially of:
A method of monitoring or detecting diabetes mellitus by determining the concentration of D-.beta.-hydroxybutyrate in a biological fluid, said method consisting essentially of:
A composition capable of exhibiting a sufficient color transition upon contacting a test sample to demonstrate the presence or concentration of D-.beta.-hydroxybutyrate in the test sample, said composition consisting essentially of:
A composition capable of exhibiting a sufficient color transition upon contacting a test sample to demonstrate the presence or concentration of D-.beta.-hydroxybutyrate in the test sample, said composition consisting essentially of:
A composition capable of exhibiting a sufficient color transition upon contacting a test sample to demonstrate the presence or concentration of D-.beta.-hydroxybutyrate in the test sample, said composition consisting essentially of:
A composition capable of exhibiting a sufficient color transition upon contacting a test sample to demonstrate the presence or concentration of D-62 -hydroxybutyrate in the test sample, said composition consisting essentially of:
Dependencies are inferred from the claim text.