Method for using polymyxin-coated substrate for lipopolysaccharide detection
OwnerBurton W. Blais, Hiroshi Yamazakiinventors
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Abstract
An improved device is provided which may be used, e.g. for immunoassay of lipopolysaccharides or for removing LPS pyrogens from aqueous solutions, or for removing LPS endotoxins from wounds. Such device comprises, in combination, a substrate, e.g. plastic, i.e. polystyrene, polycarbonate, polymethylmethacrylate or polyvinyl chloride, or a woven cloth, i.e. a rayon/polyester cloth or a polyester cloth, or a non-woven cloth, i.e. a rayon/polyester cloth, or a polyester cloth, or paper, which is adapted to receive a sample to be tested, and an oligopeptide, or a hydrophobic polypeptide or a polymyxin, e.g. polymyxin B, polymyxin B.sub.1, polymyxin B.sub.2, polymyxin D.sub.1, polymyxin D.sub.2, or polymyxin E, adhered to the substrate.
Claims12
A process for the detection of lipopolysaccharide present on the cell walls of a target Gram negative bacteria which process comprises the steps of:
- a) heating a sample of bacteria in a detergent solution to extract lipopolysaccharide antigens from said cell walls of said Gram negative bacteria;
- b) contacting said solution with a device consisting essentially of in combination, a macroporous, hydrophobic material as a substrate, said substrate comprising a cloth composed of hydrophobic synthetic polymeric fibers, selected from the group consisting of polyester, polypropylene, and nylon and blends thereof with rayon which are either woven or non-woven into a physically-structurally-stable cloth of more than about 200 .mu.m thickness, such that the pores exceed about 20 .mu.m in diameter, said substrate being adapted to receive a sample to be tested, and, bound by simple adsorption to said substrate, a polymyxin;
- c) washing said device;
- and
- d) detecting the presence of said predetermined lipopolysaccharide by contacting an antibody indicator conjugate specifically binding said lipopolysaccharide to said device.
Dependencies are inferred from the claim text.