Production of human T-cell leukemia (lymphotropic) retrovirus (HTLV-I) envelope protein fragments in bacteria and use in seroepidemiological studies
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsTakis S. Papas, Kenneth Samuel, James A. Lautenberger, Flossie Wong-Staal
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Abstract
Antigenic proteins may be expressed in bacteria by use of vectors having inserted therein DNA fragments from an envelope gene. The DNA fragments employed in the example are coding sequences found in the HTLV-I envelope gene. The bacteria used was E. coli. The antigenic proteins are useful in identifying antibodies to the organisms from which the DNA fragments were originally obtained.
Claims15
A polypeptide having the sequence of amino acids encoded by an isolated HTLV-I envelope DNA fragment contained in plasmid pKS300 having ATCC Accession No. 39902, said envelope polypeptide being immunologically reactive with anti-envelope antibodies in sera from HTLV-I infected individuals.
A polypeptide having the sequence of amino acids encoded by an isolated HTLV-I envelope gene fragment resulting from XhoI and BamHI restriction endonuclease digestion of cloned HTLV-I DNA containing the HTLV-I envelope gene, said polypeptide being immunologically reactive with anti-envelope antibodies present in sera from HTLV-I infected individuals.
A polypeptide having the sequence of amino acids encoded by an isolated HTLV-I envelope DNA fragment contained in plasmid pKS400 having ATCC Accession No. 39903, said envelope polypeptide being immunologically reactive with anti-envelope antibodies in sera from HTLV-I or HTLV-II infected individuals.
Dependencies are inferred from the claim text.