New extractive process for the recovery of naturally occurring macrolides
AssigneeAmerican Home Products Corporation
InventorConstantine Gletsos
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Abstract
This invention relates to a process of recovering a neutral macrolide antibiotic, especially a tricyclic macrolide such as rapamycin from concentrates of fermentation broth extracts or mother liquors whereby acidic and/or basic components formed in the fermentation process are removed by utilizing aqueous base or acid extraction procedures from a water-immiscible solution of said concentrate and non-polar components are separated from the neutral macrolide by selective solubility.
Claims11
A process for separating a neutral non-polypeptide macrolide from acidic, basic and non-polar neutral impurities present in a concentrate of fermentation broth extracts or mother liquors containing said neutral macrolide which comprises in any order extraction step (a) and optionally one or both of steps (b) and (c) as follows:
- (a) a solution of said concentrate in a water-immiscible solvent is extracted with aqueous base to substantially remove all acidic impurities;
- (b) a solution of said concentrate in a water-immiscible solvent is extracted with aqueous acid to substantially remove all basic impurities;
- (c) a solution of said concentrate is treated with a non-aromatic hydrocarbon solvent to separate the non-polar neutral impurities from the neutral macrolide.
Dependencies are inferred from the claim text.