P450db1 clones for identifying humans with genetic defect in drug metabolism
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsFrank J. Gonzalez, James P. Hardwick, Harry V. Gelboin, Urs A. Meyer
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Abstract
A cloned cDNA encoding debrisoquine 4-hydroxylase gene and a probe for identifying humans having a genetic defect in the metabolism of a certain class of drugs of which debrisoquine is a model, have been prepared. Testing of new drugs and of humans by a simple assay has also been described.
Claims5
An isolated and purified cDNA clone of a human P450db1 gene which encodes and expresses debrisoquine 4-hydroxylase in a mammalian cell comprising the wild-type sequence shown in FIG. 4a.
An isolated nucleotide sequence comprising a nucleotide sequence of human P450db1 as shown in FIG. 4a and encoding human debrisoquine 4-hydroxylase wherein the nucleotide sequence is selected from the group consisting of the wild-type sequence, the variant (a) sequence, the variant (b) sequence, and the variant (b') sequence.
Dependencies are inferred from the claim text.