Method of continuously preparing a sterile culture medium
AssigneeWestfalia Separator AG
InventorsKlaus Mannweiler, Ralf Kuchenbecker, Werner Rosenthal
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Abstract
A method for continuously preparing a sterile culture medium comprises combining separate unsterilized fluid components in separate streams into a single mainstream. The mainstream is applied to a transverse flow filtration module having a membrane which separates the mainstream into a permeate and a retentate. The permeate is fed to a bioreactor and the retentate is diverted to a centrifuge to precipitate contaminants. The clarified retentate is returned into the mainstream upstream of the transverse flow filtration module.
Claims9
A method for continuously preparing a sterile culture medium, comprising the steps of:
- providing unsterilized fluid components in separate streams;
- combining the separate streams into a single mainstream;
- continuously applying the mainstream to a transverse flow filtration module having a membrane which separates the mainstream into a permeate and a retentate;
- continuously feeding the permeate to a bioreactor;
- diverting the retentate to a centrifuge to continuously precipitate contaminants out of the retentate;
- and
- returning clarified retentate from the centrifuge into the mainstream upstream of the transverse flow filtration module.
Dependencies are inferred from the claim text.