Lipase immobilized on a chitosan carrier

An immobilized lipase produced by

• dissolving low-molecular weight chitosan in an aqueous acid solution and dropping the solution into a basic solution, thereby producing regenerated porous chitosan in particles,
• reacting a glycidyl ether of an aliphatic polyalcohol selected from the group consisting of ethylene glycol diglycidyl ether or polyethylene glycol diglycidyl ether, having a dimethylene ether repeating number of 1 to 22, polypropylene glycol diglycidyl ether having a propylene ether repeating number of 1 to 66 and glycerol polyglycidyl ether having 2 to 3 glycidyl ethers, with the regenerated porous chitosan in particles at a ratio of 0.01 to 0.4 mole glycidyl ether per 1 mole of the pyranose ring residue of the chitosan to crosslink the regenerated porous chitosan,
• reacting an acid halide of a higher fatty acid selected from the group consisting of lauroyl chloride, myristoyl chloride, palmitoyl chloride, stearoyl chloride, and oleyl chloride, or an acid anhydride of a higher fatty acid of C6 to C20 selected from the group consisting of lauric anhydride, myristic anhydride, palmitic anhydride, stearic anhydride, octanoyl chloride and oleic anhydride, at a ratio of 0.05 to 1 mole acid halide or acid anhydride per 1 mole of the pyranose ring residue of the chitosan to obtain an enzyme immobilizing carrier,
• and reacting a lipase and a polyfunctional cross-linking agent with the enzyme immobilizing carrier to covalently bind the lipase to the enzyme immobilizing carrier to obtain the immobilized lipase.