Enhanced attrition bioreactor for enzyme hydrolysis or cellulosic materials

A process for converting cellulosic material into glucose, comprising the steps of:

• shredding the cellulosic material to increase the surface area of the cellulosic material;
• mixing the shredded cellulosic material with a sufficient amount of water to form a slurry;
• introducing the slurry into a reaction vessel;
• introducing cellulase into the reaction vessel under conditions suitable to cause a hydrolysis reaction of the cellulose, the hydrolysis reaction forming glucose and cellobiose from the cellulose;
• circulating a first side stream from the reaction vessel through an attritor to form a second side stream, the first side stream containing water, cellulase, cellulosic material, glucose, and cellobiose, the attritor comprising a centrifugal pump, the attritor being in fluid communication with the reaction vessel, the attritor providing increased surface area to the cellulosic material;
• circulating the second side stream from the attritor through a first filter means to form a third side stream, the first filter means being in fluid communication with the attritor and the reaction vessel, the second side stream containing water, cellulase, cellulosic material, glucose, and cellobiose, the first filter means separating the cellulosic material of the second side stream from the water, cellulase, glucose and cellobiose of the second side stream, the third side stream containing water, cellulase, glucose and cellobiose;
• recycling the cellulosic material of the second side stream back to the reaction vessel;
• circulating the third side stream from the first filter means through a second filter means to form a fourth side stream, the second filter means being in fluid communication with the first filter means and the reaction vessel, the third side stream containing water, cellulase, glucose and cellobiose, the second filter means separating the cellulase of the third side stream from the water, glucose and cellobiose of the third side stream, the fourth side stream containing water, glucose and cellobiose;
• recycling the cellulase of the third side stream back to the reaction vessel;
• circulating the fourth side stream from the second filter means through a cellobiase. reactor to form a fifth side stream, the cellobiase reactor being in fluid communication with the second filter means, the cellobiase reactor comprising a fixed bed of immobilized cellobiase, the cellobiase reactor providing continuous removal of cellobiose from the fourth side stream, the cellobiase reactor converting the cellobiose of the fourth side stream to glucose, the fifth side stream containing glucose and water;
• circulating the fifth side stream from the cellobiase reactor through a third filter means to form a glucose product stream, the third filter means being in fluid communication with the cellobiase reactor and the reaction vessel, the third filter means separating the glucose of the fifth side stream from most of the water of the fifth side stream;
• and
• recycling most of the water of the fifth side stream back to the reaction vessel and removing the glucose product stream from the third filter means.