Immortalized human cell lines containing exogenous cytochrome P450 genes
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsCurtis C. Harris, Harry V. Gelboin, Frank J. Gonzalez, Katharine C. Mace, Andrea M. A. Pfeifer
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Abstract
Non-tumorigenic, stable, human bronchial and liver epithelial cell lines are provided wherein the cell lines are capable of expressing human cytochrome P450 genes which have been inserted into the cell lines. Also provided are methods and kits for identifying potential mutagens, cytotoxins, carcinogens, chemotherapeutic and chemo-preventive agents utilizing these cell lines.
Claims11
A non-tumorigenic, immortalized, human bronchial epithelial cell line containing an exogenous cytochrome P450 gene, which is expressed in said cell line;
- the cell line produced by inserting said exogenous cytochrome P450 gene into a BEAS-2B cell line, deposited as ATCC CRL 9609, or a homolog or derivative of the BEAS-2B cell line.
A non-tumorigenic, immortalized, human adult liver epithelial cell line containing an exogenous cytochrome P450 gene which is expressed in said cell line, said cell line produced by inserting said exogenous cytochrome P450 gene into a THLE-5B cell line, deposited as ATCC CRL 11113, or a homolog or derivative of the THLE-5B cell line.
Dependencies are inferred from the claim text.