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US 5,503,978 AGranted
Method for identification of high affinity DNA ligands of HIV-1 reverse transcriptase
AssigneeUniversity Research Corporation
InventorsDaniel J. Schneider, Larry Gold, Juli Feigon
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Abstract
Methods are described for the identification and preparation of DNA ligands to the HIV-1 reverse transcriptase protein. The invention encompasses specific ssDNA ligands to HIV-1 reverse transcriptase identified by the SELEX method including ssDNA ligands that inhibit the activity of HIV-1 reverse transcriptase.
Claims3
A method of identifying nucleic acid ligands to HIV-1 reverse transcriptase, comprising:
- a) preparing a candidate mixture of deoxyribonucleic acids (DNA);
- b) contacting the candidate mixture of DNA with HIV-1 reverse transcriptase, wherein nucleic acids having an increased affinity to HIV-1 reverse transcriptase relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- and
- d) amplifying the increased affinity nucleic acids to yield a ligand-enriched mixture of nucleic acids, whereby nucleic acid ligands to HIV-1 reverse transcriptase may be identified.
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Dependencies are inferred from the claim text.