Polyredox couples in analyte determinations
AssigneeMolecular Devices, LLC
InventorsHenry G. Wada, Harden M. McConnell, Dean G. Hafeman
Life of this patent
Estimating the term…
Abstract
Enzyme or substrate determinations can be achieved by employing an organic second substrate, which produces a product which may be coupled with a metal inorganic redox couple capable of interacting with and affecting the potential of a metal electrode on a semiconductor surface. Particularly, hydrogen peroxide or indoxyl phosphate are coupled with iron- or ruthenium-containing ionic redox couples for determination with a noble metal electrode.
Claims17
In a method for determining the presence of an oxidase in an assay medium by employing a metal electrode for detection of a redox reaction, the improvement which comprises:
- (a) a tetramethylbenzidine and hydrogen peroxide;
- with
- (b) an iron or ruthenium containing ionic redox couple in the assay medium.
In a method for determining the presence of peroxidase in an assay medium by employing a metal electrode for detection of a redox reaction, the improvement which comprises:
- (a) a benzidine and hydrogen peroxide;
- with
- (b) an ionic redox couple containing an element selected from the group consisting of iron and ruthenium in the assay medium.
In a method for determining the presence of an oxidase in an assay medium by employing a metal electrode for detection of a redox reaction, the improvement which comprises:
- (a) a tetramethylbenzidine and hydrogen peroxide, with
- (b) an ionic redox couple containing an element selected from the group consisting of iron and ruthenium in the assay medium.
In a method for determining the presence of a hydrolase in an assay medium by employing a metal electrode for detection of a redox reaction, the improvement which comprises:
- (a) a compound selected from the group consisting of indoxyl ester and other;
- with
- (b) an ionic redox couple containing an element selected from the group consisting of iron and ruthenium in the assay medium.
Dependencies are inferred from the claim text.