Nucleic acids encoding a cellulose binding domain
AssigneesYissum Research Development Company of the Hebrew University of Jerusalem, The Regents of the University of California
InventorsOded Shoseyov, Itai Shpiegl, Marc A. Goldstein, Roy H. Doi
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Abstract
A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.
Claims11
An isolated nucleic acid encoding a cellulose binding domain (CBD) comprising the nucleic acid sequence depicted in SEQ ID NO:
- 1 or its complement.
An isolated nucleic acid encoding a cellulose binding domain consisting of the nucleic acid sequence depicted in SEQ ID NO:
- 1 or its complement.
An isolated nucleic acid comprising nucleic acid encoding the cellulose binding domain (CBD) depicted in SEQ ID NO:
- 2 or said nucleic acid complement.
An isolated RNA polynucleotide transcribed from a nucleic acid selected from the group consisting of SEQ ID NO:
- 1, the complement of SEQ ID NO:
- 1, SEQ ID NO:
- 2, and the complement of SEQ ID NO 2.
Plasmid pET-CBD as deposited with the ATCC and assigned ATCC accession number 75444.
Recombinant E. coli pET-CBD/BL21 (DE3) as deposited with the ATCC and assigned ATCC accession number 69282.
Dependencies are inferred from the claim text.