Process of preparing a soluble LDL receptor
AssigneeYeda Research and Development Co. Ltd.
InventorsMenachem Rubinstein, Daniela Novick, Nathan Tal
Life of this patent
Estimating the term…
Abstract
A soluble LDL receptor protein is provided. It can be isolated from cells that have been treated with an interferon, isolated from the urine of healthy human individuals or produced by recombinant techniques. The soluble LDL receptor protein is useful in protection of mammals against viral infections.
Claims14
A process for the preparation of a soluble LDL receptor protein, comprising:
- (a) treating, with interferon-.gamma., cells capable of entering an antiviral state in response to induction by interferon-.gamma. to produce soluble LDL receptor protein;
- (b) isolating the soluble LDL receptor protein from the supernatant;
- and
- (c) purifying the soluble LDL receptor.
A process for preparing a soluble LDL receptor protein, comprising:
- (a) growing to confluency cells capable of entering an antiviral state in response to induction by interferon-.gamma. to produce soluble LDL receptor protein;
- (b) inducing the cells with interferon-.gamma.;
- (c) harvesting the culture supernatant;
- (d) concentrating the supernatant;
- (e) subjecting the concentrated supernatant of step (d) to anion exchange chromatography and selecting the fraction having antiviral activity;
- (f) applying the fraction obtained in step (e) to chromatography on a hydroxyapatite column and selecting the fraction having antiviral activity;
- (g) applying the fraction obtained in step (f) to anion HPLC and selecting the fraction having antiviral activity;
- (h) applying the fraction obtained in step (g) to hydrophobic interaction chromatography and selecting the fraction having antiviral activity;
- (i) applying the fraction obtained in step (h) to reverse phase HPLC and selecting the fraction having antiviral activity;
- and
- (j) repeating step (i) to obtain soluble LDL receptor purified to homogeneity.
A process for preparing a soluble LDL receptor protein from a biological fluid sample isolated from a mammalian body, comprising:
- (a) concentrating the biological fluid sample;
- (b) subjecting the concentrated sample of step (b) to anion exchange chromatography and selecting the fraction having antiviral activity;
- (c) applying the fraction obtained in step (b) to chromatography on a hydroxyapatite column and selecting the fraction having antiviral activity;
- (d) applying the fraction obtained in step (c) to anion HPLC and selecting the fraction having antiviral activity;
- (e) applying the fraction obtained in step (d) to hydrophobic interaction chromatography and selecting the fraction having antiviral activity;
- (f) applying the fraction obtained in step (e) to reverse phase HPLC and selecting the fraction having antiviral activity;
- (g) repeating step (f) to obtain soluble LDL receptor purified to homogeneity.
A process for obtaining a soluble LDL receptor protein, comprising:
- obtaining a biological fluid sample from a mammalian body;
- and
- isolating the soluble LDL receptor protein therefrom.
Dependencies are inferred from the claim text.