Growth inhibitory factor from urogenital sinus
AssigneeBaylor College of Medicine
InventorDavid R. Rowley
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Abstract
A process is provided for purifying Urogenital Sinus Derived Growth Inhibitory Factor (UGIF) from embryonic tissue which comprises chromatographing medium from cultures or culture-derived spheroids of embryonic tissue derived from the urogenital sinus by gel filtration chromatography. Further purification by reverse phase high pressure liquid chromatography is also demonstrated. The UGIF is obtained in 70-fold to 8000-fold purification over the conditioned medium. A UGIF composition of matter is also provided, as is a method for treating neoplasia with UGIF.
Claims6
A pharmaceutical composition comprising urogenital sinus derived inhibitory factor having an activity of at least 1 as measured by the UGIF units assay test, being substantially free of albumin and being about 70-8000 fold enriched in UGIF activity over tissue per dry weight of tissue.
A pharmaceutical composition comprising urogenital sinus derived inhibitory factor, having a molecular weight of about 10,000-20,000 Daltons, retaining biological activity and acid stability following treatment at 80.degree. C. for 10 min., wherein said composition inhibits the growth of Y-79, A-549, Mv 1 Lu, NRK, NBT-11 and Hela cells.
A pharmaceutical composition comprising urogenital sinus inhibitory factor having a specific activity greater that 70 units per mg protein.
Dependencies are inferred from the claim text.