Processes for producing pre-prorennin, prorennin and rennin
AssigneeGenome Therapeutics Corporation
InventorsBernadette L. Alford, Jen-I Mao, Donald T. Moir, Alison Taunton-Rigby, Gerald F. Vovis
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Abstract
Living cells containing genetic material derived from recombinant DNA material and capable of expressing rennin, pre-prorennin and prorennin. The rennin, pre-prorennin and prorennin are derived from cells which are themselves or have had parents thereof treated by recombinant DNA methods to allow production of the desired enzymatic proteins during growth in culture.
Claims55
A pre-prorennin preparation substantially free of other proteolytic protein found in the stomach of a pre-ruminant calf, produced by a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for pre-prorennin.
A process for producing pre-prorennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence code for pre-prorennin, and obtaining pre-prorennin from said cell.
A process for producing prorennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast ad bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for prorennin, and obtaining prorennin from said cell.
A process for producing rennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for rennin, and obtaining rennin from said cell.
A process for producing rennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for pre-prorennin, obtaining pre-prorennin from said cell and cleaving said pre-prorennin to produce rennin.
A process for producing rennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for prorennin, obtaining prorennin from said cell and cleaving said prorennin to produce rennin.
A process for producing rennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for prorennin;
- and obtaining rennin therefrom.
A process for producing rennin which comprises culturing a transformed living cell selected from the group consisting of fungi, yeast and bacteria, containing and capable of expressing genetic material derived from recombinant DNA material comprising a nucleotide sequence coding for preprorennin;
- and obtaining rennin therefrom.
Dependencies are inferred from the claim text.