Assay method for hemicellulases using a colored substrate
AssigneesCiba-Geigy Corporation, Genencor International, Inc.
InventorsBeat Freiermuth, Dieter Werthemann, Alfred Gaertner, Spencer Fisk
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Abstract
The present invention provides an assay method for hemicellulases comprising a) directly dyeing, using a reactive dye, an insoluble natural product, or a modified form of a natural fibre material; and b) adding the enzyme to the dyed product produced in step a) and, after a specific incubation period, separating the liquid component from the insoluble dyed product, e.g. by a simple filtration, and determining the amount of dyestuff liberated in the the separated solution by spectrophotometric means. A combined mixing and dispensing device for use in the method of the present invention is also provided.
Claims13
In an assay method for hemicellulases having the steps of a) dyeing, using a reactive dye, wood or other vegetable material that has been mechanically decomposed or homogenized;
- and b) adding an enzyme to the dyed material produced in step a) and, after an incubation period of from 2 minutes to 4 hours, separating a liquid component from the dyed material and determining the amount of dye liberated in the separated liquid component by spectrophotometric means, the improvement comprising dyeing insoluble wood or other vegetable material that has been mechanically decomposed or homogenized in step a) and, in step b), after incubation, separating the liquid component from the dyed material without the use of an organic solvent.
Dependencies are inferred from the claim text.