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US 5,496,705 AGranted
Monoclonal antibody specific for rat synaptophysin
AssigneeKabushiki Kaisha Toshiba
InventorMitsuko Sugano
Life of this patent
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Abstract
An object of this invention is to provide a monoclonal antibody which can quantitatively analyze a very small amount of synaptophysin present in tissues using a small amount of the antibody in accordance with a complement fixation test. Disclosed is a monoclonal antibody produced by using a rat brain extract as an immunogen, wherein an immunoglobulin class thereof is IgM, and the monoclonal antibody has specific reactivity for rat synaptophysin or P-38.
Claims8
A method of detecting a neuroendocrine tumor, comprising:
- contacting (a) a labeled monoclonal antibody produced by the hybridoma RB2-4, deposited with the Fermentation Research Institute and assigned the Deposit No. FERM BP-3944, said monoclonal antibody being of the immunoglobulin class M and specifically binding rat synaptophysin, or (b) a labeled monoclonal antibody having the identifying characteristics of the monoclonal antibody produced by said hybridoma, with an organ, a tissue slice or a tissue homogenate which may contain synaptophysin, to bind said labelled monoclonal antibody to synaptophysin;
- and
- correlating the presence or concentration of said monoclonal antibody bound to said synaptophysin to an indication of a neuroendocrine tumor.
A method of effecting or quantitatively analyzing rat synaptophysin, comprising:
- performing an immunoassay by contacting a rat tissue homogenate with (a) a monoclonal antibody produced by the hybridoma RB2-4, deposited with the Fermentation Research Institute and assigned the Deposit No. FERM BP-3944, said monoclonal antibody being of the immunoglobulin class M and specifically binding rat synaptophysin, or (b) a monoclonal antibody having the identifying characteristics of the monoclonal antibody produced by said hybridoma, and detecting said monoclonal antibody specifically bound to said rat tissue homogenate to obtain a result of said immunoassay;
- and
- correlating said result of said immunoassay to the presence or quantity of said rat synaptophysin.
Dependencies are inferred from the claim text.