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US 5,496,473 AGranted
Apparatus for processing biopolymer-containing columns
AssigneeBarrskogen, Inc.
InventorFlora Chow
Life of this patent
Estimating the term…
Abstract
An apparatus has been designed that is useful for the automated or semi-automated removal, recovery, deprotection, and/or purification of biopolymers such as oligodeoxyribonucleotides, oligoribonucleotides, oligosaccharides, and peptides from a solid support matrix that is contained in a reaction chamber in the shape of a column.
Claims13
An apparatus for the automated delivery of a liquid through a substantially cylindrical column containing solid matrix, the apparatus comprising,
- (a) a substantially cylindrical column, comprising a solid matrix, said column having, at opposite ends thereof, an inlet port and an outlet port, said inlet port being in fluid communication with said delivery means,
- (b) syringe-like liquid delivery means comprising a movable plunger within a cylindrical barrel, said delivery means being capable of receiving and holding said liquid and being in fluid communication with said inlet port of said column, and capable of delivering said liquid into said inlet port of said column upon depression of said plunger,
- (c) liquid receiving means in fluid communication with said outlet port of said column,
- (d) drive means capable of depressing said plunger in a controlled fashion, comprising;
- (i) plunger engagement means for contacting, in order to depress, said plunger,
- (ii) linkage means capable of moving said plunger engagement means in response to the operation of a motor, and
- (iii) a motor capable of moving said engagement means at a controlled rate to depress the plunger, thereby delivering said liquid to said column, and
- (e) support means capable of retaining said drive means in operational alignment with said delivery means, and capable of releasably receiving and supporting said barrel, said column, and said receiving means in axial alignment with each other.
5 more elements
A process for the removal, recovery, deprotection, and/or purification of biopolymers from a solid matrix, the process comprising the steps of:
- (a) providing a substantially cylindrical column, comprising a biopolymer attached to a solid matrix, said column having, at opposite ends thereof, an inlet port and an outlet port, said inlet port being in fluid communication with said delivery means,
- (b) providing an apparatus comprising
- (i) syringe-like liquid delivery means comprising a movable plunger within a cylindrical barrel, said delivery means being capable of receiving and holding said liquid and being capable of delivering said liquid into said inlet port of said column upon depression of said plunger,
- (ii) liquid receiving means in fluid communication with said outlet port of said column,
- (iii) drive means capable of depressing said plunger in a controlled fashion, comprising;
- a. plunger engagement means for contacting, in order to depress, said plunger,
- b. linkage means capable of moving said plunger engagement means in response to the operation of a motor, and
- c. a motor capable of moving said engagement means at a controlled rate to depress the plunger, thereby delivering said liquid to said column, and
- (iv) support means capable of retaining said drive means in operational alignment with said delivery means, and capable of releasably receiving and supporting said barrel, said column, and said receiving means in axial alignment with each other,
- (c) attaching said column to said apparatus in order to provide fluid communication between said delivery means and said inlet port of said column, and between said receiving means and said outlet port of said column,
- (d) sequentially delivering desired liquids useful for the removal, recovery, deprotection, and/or purification of said biopolymer through said column from said delivery means, and
- (d) recovering desired fractions of said delivered liquids in said receiving means.
9 more elements
Dependencies are inferred from the claim text.