Method of preserving spermatozoa
OwnerCarl O. Gray, Jr.inventor
Life of this patent
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Abstract
Applicant has found that by freezing spermatozoa, especially stallion spermatozoa, in an extender in a flat container having a thickness so that the frozen product does not exceed 4 mm thickness results in a significant increase in the number of spermatozoa surviving upon thawing.
Claims4
A method of preserving spermatozoa which comprises placing a mixture of spermatozoa and a cryoprotectant for said spermatozoa in a flexible flat container, and thereafter freezing said container under restraints so that the thickness of said container, when frozen, will not exceed 4 millimeters.
A method of preserving spermatozoa which comprises placing a mixture of spermatozoa and a cryoprotectant for said spermatozoa in a flexible flat container, and thereafter squeezing said flat container between metal grids supported by a material having sufficient buoyancy to support said metal grids and said flat container on liquid nitrogen, thereafter floating said combination of said flat container and said metal grids and said buoyant material on liquid nitrogen for at least 15 minutes or until said spermatozoa are frozen.
Dependencies are inferred from the claim text.