Insect resistant tomato and potato plants
AssigneeMonsanto Company
InventorsDavid A. Fischhoff, Roy L. Fuchs, Paul B. Lavrik, Sylvia A. McPherson, Frederick J. Perlak
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Abstract
A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.
Claims20
A chimeric gene capable of expressing in a plant cell comprising in sequence:
- (a) a promoter which functions in plants to cause the production of RNA;
- (b) a DNA sequence that causes the production of a RNA sequence encoding Coleopteran-type toxin protein of Bacillus thuringiensis var. tenebrionis having the amino acid sequence selected from the group consisting of from residues (1-644), residues (16-644), residues (48-644), residues (50-644), residues (58-644) and residues (77-644) of said protein wherein the amino acid residues of said protein are numbered as shown in FIG. 10;
- and
- (c) a 3' non-translated DNA sequence which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence.
A chimeric gene capable of expressing in a plant cell comprising in sequence:
- (a) a promoter which functions in plants to cause the production of RNA;
- (b) a DNA sequence that causes the production of a RNA sequence encoding Coleopteran-type toxin protein of Bacillus thuringiensis var. tenebrionis having the amino acid sequence from residues (48-644) of said protein wherein the amino acid residues of said protein are numbered as shown in FIG. 10;
- and
- (c) a 3' non-translated DNA sequence which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence.
A DNA sequence that encodes a Coleopteran-type toxin protein of Bacillus thuringiensis var. tenebrionis which is effective in controlling Coleopteran-type insects having the amino acid sequence selected from the group consisting of from residues (16-644), residues (48-644), residues (50-644), residues (58-644) and residues (77-644) of said protein wherein the amino acid residues of said protein are numbered as shown in FIG. 10.
A DNA sequence that encodes a Coleopteran-type toxin protein of Bacillus thuringiensis var. tenebrionis which is effective in controlling Coleopteran-type insects having the amino acid sequence from residues (48-644) of said protein wherein the amino acid residues of said protein are numbered as shown in FIG. 10.
A transformed plant cell expressing the toxin protein of Bacillus thuringiensis var. tenebrionis having the amino acid sequence from residues (48-644) of the full-length protein wherein the amino acid residues of said full-length protein are numbered as shown in FIG. 10.
A transformed plant selected from the group consisting of tomato and potato expressing the toxin protein of Bacillus thuringiensis var. tenebrionis having the amino acid sequence from residues (48-644) of the full-length protein wherein the amino acid residues of said full-length protein are numbered as shown in FIG. 10.
A substantially pure toxin protein of Bacillus thuringiensis var. tenebrionis having the amino acid sequence from residues (48-644) of the full-length protein wherein the amino acid residues of said full-length protein are numbered as shown in FIG. 10.
A toxin protein of Bacillus thuringiensis var. tenebrionis free of other proteins of Bacillus thuringiensis var. tenebrionis said toxin protein having the amino acid sequence from residues (48-644) of the full-length protein wherein the amino acid residues of said full-length protein are numbered as shown in FIG. 10.
Dependencies are inferred from the claim text.