Oligonucleotide probes for detection of salmonella
AssigneeAmoco Corporation
InventorsDavid J. Lane, Ayoub Rashtchian, Kyriaki Parodos
Life of this patent
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Abstract
Nucleic acid fragments capable of hybridizing to rRNA of a Salmonella species and not capable of hybridizing to rRNA of Escherichia coli.
Claims11
A probe of at least 25 nucleotides in length, said probe consisting of a nucleic acid sequence that is complementary or identical to any 25 or more consecutive nucleotides of the S. typhimurium 23S rRNA subunit between nucleotides 1474 and 1527, the S. arizona 16S rRNA subunit between nucleotides 443 and 491, the S. typhimurium 16S rRNA subunit between nucleotides 991 and 1045, or the 16S rRNA subunit of CDC stk. N55 1925 between nucleotides 443 and 491.
A probe consisting of a nucleic acid with the following sequence:
- TCTGTGCATCCACTTCACTAAATGAGTAC-5';
- TCCACACACAAGGTCCATTTAGGCCAAGTGAAATTGTGC-5';
- CCCCCTCCTTCCACAACACCAATTATTGGCGTCGTTAACTGCAAT-5';
- TCCTTCCACAACACCAATTATTGGCGTCGTTAACT-5';
- TCCTTCCCCTATTCCGATTATTGGAACAAGTAACT-5';
- ACTTCGCCAAATGGGCACCTCGACTTCAGTCAGCT-5';
- GGTGTCTTCAAACGTCTCTACGCTTACACGGAAGC-5';
- GGTGTCTTGAAAGGTCTCTACCTAACCACGGAAGC-5';
- TCCACAACACCAATTATTGGCGTCGTTAAC-5';
- CTCTTCCGCTGTTCCAATTATTGGAACAACTAACT-5';
- CGACCAAAAGGTCCGTTTAGGCCTTTTAGTTCCG-5';
- TGTACATCCACTTCGCCAAATGGGCAC-5';
- TCGCCAAATGGGCACCTCGACTTCAGT-5';
- CTGTGCATCCACTTCACCAAATGGGTA-5';
- AAAGTCGCCCCTCCTTCCACAACACCAATT-5';
- ATTGGCGTCGTTAACTGCAATGGGCGTCTT-5';
- CGTGTCCAAATGGACACACTGACGCATG-5';
- or
- CACCCTCGTGTCCAAATGGACACACTGA-5'.
Dependencies are inferred from the claim text.