Electrochemical immunosensor system and methods
AssigneeHughes Aircraft Company
InventorAndrew Kindler
Life of this patent
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Abstract
A electrochemical immunosensor system is provided which uses electrical signals to measure binding events. The system includes an immunosensor having a sensing electrode in contact with a fluid containing an analyte concentration. A quantity of antibodies or other binding agent is adsorbed on or otherwise affixed to the electrode surface such that a portion of the antibodies of the binding agent binds a portion of the analyte to form complexes on the electrode surface. Signal generating means develop an electrical signal at the sensing electrode such that a response current is produced through the sensing electrode. The response current has measurable signal characteristics which are dependent upon the number of complexes formed, and therefore the analyte concentration within the fluid.
Claims12
An electrochemical immunosensor system adapted for use in measuring a concentration of analytes in a fluid, said system comprising:
- an immunosensor having at least one sensing electrode for contact with said fluid, said sensing electrode being constructed of a material selected from the group consisting of gold and platinum and having a surface on which a binding agent consisting of antibodies is present, such that a portion of said binding agent binds a portion of said analyte in said fluid to form a number of complexes;
- signal generating means electrically connected to said sensing electrode for generating an electrical signal for application to said sensing electrode comprising:
- a waveform generator which provides an ac signal;
- a potentiostat having an input to which said ac signal is applied and an output electrically connected to said sensing electrode;
- and,
- a dc signal generator which provides a dc sweep signal, wherein said electrical signal at said sensing electrode includes said ac signal superimposed on said dc signal
- wherein said electrical signal produces a response current having at least one signal characteristic which is dependent upon said number of complexes;
- and
- signal monitoring means electrically connected to said response current for monitoring said signal characteristic of said response current and comprising phase-locking means for tracking potions of said response current that are at a specified phase angle with respect to a reference signal.
A method of measuring an analyte concentration using an immunosensor, said method comprising the steps of;
- providing a fluid containing an analyte concentration to be measured;
- providing an immunosensor having at least one sensing electrode, said sensing electrode having a surface on which a binding agent consisting of antibodies is present;
- placing said sensing electrode of said immunosensor in contact with said fluid such that a portion of said binding agent on said sensing electrode surface binds a portion of said analyte in said fluid to form a number of complexes;
- developing an electrical signal at said sensing electrode on which said complexes are formed, said electrical signal comprising a voltametric signal including an ac signal superimposed on a dc sweep signal, said ac signal having an amplitude and a frequency and said electrical signal producing a response current flowing through said sensing electrode having at least one signal characteristic which is dependent upon said number of complexes;
- and
- monitoring said signal characteristic of said response current by measuring an ac component of said response current as said dc signal is swept over a potential range, said measurement of said ac component of said response current in relation to said dc potential range being expressed as ac current spectra wherein said analyte concentration is determined from said ac current spectra.
A method of measuring an analyte concentration using an immunosensor, said method comprising the steps of:
- providing a fluid containing an analyte concentration to be measured and a quantity of indicator ions wherein said ions provide for an ionic current;
- providing an immunosensor having at least one sensing electrode, said sensing electrode having a surface on which a binding agent consisting of antibodies attached to gold particles is present wherein said binding agent forms a quantity of ion gates on said surface of said sensing electrode;
- placing said sensing electrode of said immunosensor in contact with said fluid such that a portion of said binding agent on said sensing electrode surface binds a portion of said analyte in said fluid to form a number of complexes;
- developing an electrical signal at said sensing electrode on which said complexes are formed, said electrical signal being produced by an impedance bridge and producing an ionic response current flowing through said sensing electrode having at least one signal characteristic which is dependent upon said number of complexes wherein said complexes close one or more of said ion gates and thereby reduce a magnitude of said ionic response current;
- and
- monitoring said signal characteristic of said ionic response current by means of said impedance bridge to determine said analyte concentration.
Dependencies are inferred from the claim text.