Defined medium OMPC fermentation process
AssigneeMerck & Co., Inc.
InventorJeffrey Fu
Life of this patent
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Abstract
A new fermentation process for the culture of Neisseria meningitidis incorporates a wholly synthetic medium and event based processing decisions. This process is capable of generating cells at production scale for the extraction and purification of outer membrane protein complex (OMPC) vesicles which are useful as a protein carrier for PedvaxHIB.RTM. and pneumococcal conjugate vaccines.
Claims7
A defined medium, named MC.6, which comprises, (.+-.10%) the following components (mg/L):
- ______________________________________ Component MC.6 ______________________________________ NaCl 5800 K.sub.2 HPO.sub.4 4000 NH.sub.4 Cl 1000 K.sub.2 SO.sub.4 1000 Glucose 10000 L-Glutamic Acid 3900 L-Arginine 150 Glycine 250 L-Serine 500 L-Cysteine.HCl 100 MgCl.sub.2.6H.sub.2 O 400 CaCl.sub.2.2H.sub.2 O 28 Fe(III) Citrate 40. ______________________________________
A method for culturing Neisseria meningitidis cells to an optical density of about 10-13 at 600 nm, which comprises culturing Neisseria meningitidis cells in a medium containing only the five amino acids Glu, Arg, Gly, Ser and Cys, salts and a carbon source, and harvesting the cells in late log or early stationary phase.
Dependencies are inferred from the claim text.