DNA and vectors encoding the parathyroid hormone receptor, transformed cells, and recombinant production of PTHR proteins and peptides
AssigneeThe General Hospital Corporation
InventorsGino V. Segre, Henry M. Kronenberg, Abdul-Badi Abou-Samra, Harald Juppner, John T. Potts, Jr., Ernestina Schipani
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Abstract
DNA encoding a parathyroid hormone receptor; production and isolation of recombinant and synthetic parathyroid hormone receptor polypeptides and fragments; antibodies to parathyroid hormone receptors and receptor fragments; methods for screening candidate compounds for antagonistic or agonistic effects on parathyroid hormone receptor action; and diagnostic and therapeutic methods of these compounds are disclosed.
Claims25
Isolated double- or single-stranded DNA comprising
- (1) a DNA sequence encoding a naturally occurring parathyroid hormone receptor of a mammal, or
- (2) the complement of said DNA sequence, said receptor having an amino acid sequence with at least 50% identity to the amino acid sequence encoded by SEQ ID NO:
- 3,
- wherein a single-stranded form of said isolated DNA hybridizes under stringent conditions to a hybridization probe consisting of an 18-nucleotide portion of a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and a sequence complementary to one of SEQ ID NO;
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4.
A purified preparation of a vector, said vector comprising a DNA sequence encoding a naturally occurring mammalian parathyroid hormone receptor, wherein a single-stranded form of said DNA sequence hybridizes under stringent conditions to a hybridization probe consisting of an 18-nucleotide portion of a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and a sequence complementary to one of SEQ ID NO:
- 1, SEQ ID NO. 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4.
A method for producing a polypeptide, said method comprising:
- providing a cell comprising isolated DNA encoding a naturally occurring mammalian parathyroid hormone receptor;
- culturing said cell under conditions permitting expression of said polypeptide from said DNA;
- and
- obtaining said polypeptide, provided that said DNA comprises a strand which hybridizes under stringent conditions to a hybridization probe consisting of an 18-nucleotide portion of a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and a sequence complementary to one of SEQ ID NO:
- 1, SEQ ID. NO:
- 2, SEO ID NO:
- 3, or SEO ID NO:
- 4.
Isolated double- or single-stranded DNA comprising
- (1) a DNA sequence encoding a naturally occurring parathyroid hormone receptor of a vertebrate animal, or
- (2) the complement of said DNA sequence, said receptor having an amino acid sequence with at least 50% identity to the amino acid sequence encoded by SEQ ID NO:
- 3,
- wherein a single-stranded form of said isolated DNA hybridizes under stringent conditions to DNA having a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and a sequence complementary to one of SEQ ID NO:
- 1, SEQ ID NO:
- 2. SEQ ID NO:
- 3, or SEQ ID NO:
- 4.
A purified preparation of a vector, said vector comprising a DNA sequence encoding a naturally occurring parathyroid hormone receptor of a vertebrate animal, wherein a single-stranded form of said DNA sequence hybridizes under stringent conditions to DNA having a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and a sequence complementary to one of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4.
Isolated DNA encoding a peptide selected from the group consisting of RP1 (SEQ ID NO:
- 5), RP2 (SEQ ID NO:
- 6), RP3 (SEQ ID NO:
- 7), RP4 (SEQ ID NO:
- 8), RP5 (SEQ ID NO:
- 9),and RP6 (SEQ ID NO:
- 10).
Dependencies are inferred from the claim text.