Microorganism antigen extraction methods
AssigneeBiostar, Inc.
InventorsGregory R. Bogart, Robert J. Bilodeau, Rachel M. Ostroff, Jeffrey W. Steaffens
Life of this patent
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Abstract
Method for extracting an antigen from a micro-organism organism of the genus streptococcus contained in a sample including the steps of contacting the sample with reagents which generate hyponitrous acid, further contacting the sample with a base, or contacting the sample with hypochlorite, and neutralizing the sample.
Claims44
Method for extracting an antigen from a micro organism of the genus streptococcus contained in a sample comprising the steps of:
- first contacting the sample with reagents which generate nitrous acid for a period of time sufficient to allow the nitrous acid to extract said antigen so as to result in a sample comprising extracted antigen,
- second contacting the sample comprising extracted antigen with base for a period of time sufficient to increase the exposure of the antigen so as to result in a sample comprising antigen of increased exposure, and
- neutralizing the sample comprising antigen of increased exposure.
Method for extracting an antigen from a micro organism of the genus streptococcus contained in a sample and lysing mucus in the sample, comprising the steps of:
- first contacting the sample with reagents which generate nitrous acid for a period of time sufficient to allow the nitrous acid to extract the antigen so as to result in a sample comprising extracted antigen;
- second contacting the sample comprising extracted antigen with base for a period of time sufficient to increase the exposure of the antigen and to lyse mucus in the sample comprising extracted antigen, so as to result in a sample comprising antigen of increased exposure and lysed mucus;
- and
- neutralizing the sample comprising antigen of increased exposure and lysed mucus.
Method for extracting an antigen from a micro organism of the genus streptococcus contained in a sample, comprising the steps of:
- contacting the sample with hypochlorite, and sodium nitrite for a period of time sufficient to extract the antigen so as to result in a sample comprising extracted antigen, and
- neutralizing said sample comprising extracted antigen.
Method for extracting an antigen from a micro organism of the genus streptococcus contained in a sample comprising the steps of:
- first contacting the sample with hypochlorite and sodium nitrite;
- incubating for a period of time sufficient to allow the hypochlorite to extract the antigen so as to result in a sample comprising extracted antigen;
- contacting the sample comprising extracted antigen with reagents which generate nitrous acid for a period of time sufficient to increase the exposure of the antigen so as to result in a sample comprising antigen of increased exposure;
- and
- neutralizing said sample comprising antigen of increased exposure.
Method for lysing mucus contained in a sample comprising an antigen from a microorganism of the genus streptococcus, comprising the steps of:
- contacting the sample with hypochlorite and sodium nitrite for a period of time sufficient to lyse mucus in the sample so as to result in a sample comprising lysed mucus;
- and
- neutralizing the sample comprising lysed mucus.
Kit for the extraction of antigens from micro organisms of the genus streptococcus comprising;
- extraction tubes containing dried sodium nitrite;
- an aliquot of acetic acid solution;
- an aliquot of strong base solution;
- and
- an aliquot of neutralizing buffer.
Kit for the extraction of antigens from micro organisms of the genus streptococcus comprising;
- extraction tubes containing sodium nitrite;
- an aliquot of hypochlorite solution;
- and
- an aliquot of a neutralizing buffer.
The kit of claim 21, wherein an aliquot of acetic acid solution is also included.
Method for detection of an antigen extracted from a micro organism of the genus streptococcus contained in a sample comprising the steps of:
- first contacting the sample with reagents which generate nitrous acid for a period of time sufficient to allow the nitrous acid to extract the antigen so as to result in a sample comprising extracted antigen;
- second contacting the sample comprising extracted antigen with base for a period of time sufficient to increase exposure of the antigen so as to result in a sample with antigen of increased exposure;
- neutralizing the sample comprising antigen of increased exposure so as to result in a neutralized sample;
- third contacting the neutralized sample with a test surface which specifically binds said antigen;
- and
- detecting the binding of said antigen to said test surface by a suitable analytical method.
Method for detection of an antigen extracted from a micro organism of the genus streptococcus contained in a sample comprising the step of:
- first contacting the sample with hypochlorite for a period of time sufficient to extract the antigen so as to result in a sample comprising extracted antigen,
- neutralizing the sample comprising extracted antigen so as to result in a neutralized sample,
- second contacting the neutralized sample with a test surface which specifically binds said antigen, and
- detecting the binding of said antigen by a suitable analytical method.
Assay kit for the detection of antigens extracted from micro organisms of the genus streptococcus comprising:
- extraction tubes containing dried sodium nitrite;
- an aliquot of hypochlorite solution;
- an aliquot of acetic acid solution,
- an aliquot of a neutralizing buffer;
- an aliquot of secondary label;
- an aliquot of precipitating substrate;
- and
- a test surface.
Assay kit for the detection of antigens extracted from micro organisms of the genus streptococcus comprising:
- extraction tubes containing dried sodium nitrite;
- an aliquot of acetic acid solution,
- an aliquot of a neutralizing buffer;
- an aliquot of secondary label;
- an aliquot of precipitating substrate;
- an aliquot of a solution of strong base;
- and
- a test surface.
Method for extracting an antigen from a micro organism of the genus streptococcus contained in a sample, comprising the steps of:
- contacting the sample with hypochlorite for a period of time sufficient to extract the antigen so as to result in a sample comprising extracted antigen;
- and
- neutralizing said sample comprising extracted antigen.
Method for lysing mucus contained in a sample comprising an antigen from a micro organism of the genus streptococcus, comprising the steps of:
- contacting the sample with hypochlorite for a period of time sufficient to lyse mucus in the sample so as to result in a sample comprising lysed mucus;
- and
- neutralizing the sample comprising lysed mucus.
Kit for extraction of antigens from micro organisms of the genus streptococcus comprising:
- an aliquot of hypochlorite solution;
- and
- an aliquot of a neutralizing buffer.
Assay kit for the detection of antigens extracted from micro organisms of the genus streptococcus comprising:
- an aliquot of hypochlorite solution;
- an aliquot of neutralizing buffer;
- an aliquot of secondary label;
- an aliquot of precipitating substrate;
- an aliquot of wash solution;
- and
- a test surface.
Dependencies are inferred from the claim text.