Detection and identification of mycobacteria
AssigneeBecton, Dickinson and Company
InventorsPatricia A. Spears, Michael C. Little, Daryl D. Shank
Life of this patent
Estimating the term…
Abstract
Genus- and species-specific oligonucleotide probes derived from the M. paratuberculosis 70 kD heat shock protein gene sequence. The probes are useful for detecting Mycobacteria and for identifying specific species of Mycobacteria.
Claims22
An oligonucleotide for detecting nucleic acid of a mycobacterium selected from the group consisting of M. africanum, M. avium, M. bovis, M. bovis-BCG, M. chelonae, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. scrofulaceum and M. tuberculosis, the oligonucleotide having a sequence selected from the group consisting of SEQ ID NO:14 and SEQ ID NO:15.
An oligonucleotide for detecting nucleic acid of a mycobacterium selected from the group consisting of M. avium and M. tuberculosis, the oligonucleotide having a sequence selected from the group consisting of SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:22.
An oligonucleotide for detecting nucleic acid of a mycobacterium selected from the group consisting of M. africanum, M. avium, M. bovis, M. chelonae, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. scrofidaceum and M. tuberculosis, the oligonucleotide having a sequence selected from the group consisting of SEQ ID NO:17 and SEQ ID NO:18.
An oligonucleotide for detecting nucleic acid of a mycobacterium selected from the group consisting of M. avium, M. gordonae, M. intracellulare, M. kansasii and M. tuberculosis, the oligonucleotide having a sequence selected from the group consisting of SEQ ID NO:35 and SEQ ID NO:36.
A method for amplifying nucleic acid of a mycobacterium selected from the group consisting of M. avium and M. tuberculosis, the method comprising the steps of:
- a) hybridizing to the mycobacterial nucleic acid a pair of amplification primers, the pair of amplification primers consisting of i) a first primer comprising the primer binding sequence of SEQ ID NO:20 and a second primer comprising the primer binding sequence of SEQ ID NO:21, or ii) a third primer comprising the primer binding sequence of SEQ ID NO:20 and a fourth primer comprising the primer binding sequence of SEQ ID NO:22, and;
- b) amplifying a segment of the mycobacterial nucleic acid defined by the hybridized pair of amplification primers by extending the hybridized primers on the segment of mycobacterial nucleic acid to produce an amplification product.
A method for amplifying nucleic acid of a mycobacterium selected from the group consisting of M. africanum, M. avium, M. bovis, M. chelonae, M. fortuitum, M. gordonae, M. intracelllulare, M. kansasii, M. scrofulaceum and M. tuberculosis, the method comprising the steps of:
- a) hybridizing to the mycobacterial nucleic acid a pair of amplification primers consisting of a first primer comprising the primer binding sequence of SEQ ID NO:17 and a second primer comprising the primer binding sequence of SEQ ID NO:18, and;
- b) amplifying a segment of the mycobacterial nucleic acid defined by the hybridized pair of amplification primers by extending the hybridized primers on the segment of mycobacterial nucleic acid to produce an amplification product.
A method for amplifying nucleic acid of a mycobacterium selected from the group consisting of M. avium, M. gordonae, M. intracellulare, M. kansasii and M. tuberculosis, the method comprising the steps of:
- a) hybridizing to the mycobacterial nucleic acid a pair of amplification primers consisting of a first primer comprising the primer binding sequence of SEQ ID NO:35 and a second primer comprising the primer binding sequence of SEQ ID NO:36, and;
- b) amplifying a segment of the mycobacterial nucleic acid defined by the hybridized pair of amplification primers by extending the hybridized primers on the segment of mycobacterial nucleic acid to produce an amplification product.
Dependencies are inferred from the claim text.