Loading patent…
AssigneesMassachusetts Institute of Technology, The Penn State Research Foundation
InventorsSmadar Cohen, Carmen Bano, Karyn B. Visscher, Marie Chow, Harry R. Allcock, Robert S. Langer
Estimating the term…
A method for encapsulating biologically-labile materials such as proteins, liposomes, bacteria and eucaryotic cells within a synthetic polymeric capsule, and the product thereof, are disclosed. The method is based on the use of a water-soluble polymer with charged side chains that are crosslinked with multivalent ions of the opposite charge to form a gel encapsulating biological material, that is optionally further stabilized by interactions with multivalent polyions of the same charge as those used to form the gel. In the preferred embodiment, hydrolytically stable polyphosphazenes are formed of monomers having carboxylic acid side groups that are crosslinked by divalent or trivalent cations such as Ca.sup.2+ or Al.sup.3+, then stabilized with a polycation such as poly-L-lysine. A variety of different compositions can be formed from the crosslinked polymer. In a preferred embodiment, microcapsules are made by spraying an aqueous solution of polyphosphazene and material to be encapsulated into a calcium chloride solution.
A composition in the form of a a microsphere or microcapsule formed by ionically crosslinking a synthetic polymer selected from the group consisting of poly(phosphazenes), poly(acrylic acids), poly(methacrylic acids), copolymers of acrylic acid or methacrylic acid and polyvinyl ethers or poly(vinyl acetate), sulfonated polystyrene, poly(vinyl amines), poly(vinyl pyridine), poly(vinyl imidazole), imino substituted polyphosphazenes, and ammonium or quaternary salts thereof, wherein the polymer is soluble prior to crosslinking in an aqueous solution selected from the group consisting of water, aqueous alcohol, and buffered aqueous salt solutions, with ions selected from the group consisting of calcium, copper, aluminum, magnesium, strontium, barium, organic cations, dicarboxylic acids, sulfate ions and carbonate ions.
A method for synthesizing a synthetic polymeric crosslinked composition in the form of a microsphere or microcapsule, the method comprising:
Dependencies are inferred from the claim text.