C-terminally truncated dengue and Japanese encephalitis virus envelope proteins

A vaccine composition for humans and animals against dengue virus or Japanese Encephalitis Virus (JEV) infection comprising a recombinantly expressed truncated flavivirus envelope protein, said truncated flavivirus envelope protein selected from the group consisting of:

• amino adds 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• a peptide from dengue type 1, type 2 or type 3 corresponding to amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• and
• amino acids 1-398, 1-399, 1-400, 1-401 or 1-402 of Japanese encephalitis virus (amino acids 398-402 are set forth in FIG. 11),
• wherein said envelope protein is present in said composition in an amount sufficient to induce immunity against said infection;
• and wherein said composition further comprises a pharmaceutically acceptable carrier.

A DNA construct consisting essentially of a vector and

• a DNA segment encoding a C-terminally truncated dengue virus or Japanese encephalitis virus (JEV) envelope protein, said truncated flavivirus envelope protein selected from the group consisting of:
• amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• a peptide from dengue type 1, type 2 or type 3 corresponding to amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• and
• amino acids 1-398, 1-399, 1-400, 1-401 or 1-402 of Japanese encephalitis virus (amino acids 398-402 are set forth in FIG. 11).

A recombinant virus encoding a C-terminally truncated dengue virus or Japanese encephalitis virus (JEV) envelope protein, said truncated flavivirus envelope protein selected from the group consisting of:

• amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• a peptide from dengue type 1, type 2 or type 3 corresponding to amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• and
• amino acids 1-398, 1-399, 1-400, 1-401 or 1-402 of Japanese encephalitis virus (amino acids 398-402 are set forth in FIG. 11).

A recombinant, C-terminally truncated dengue virus or Japanese encephalitis virus (JEV) envelope protein selected from the group consisting of:

• amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• a peptide from dengue type 1, type 2 or type 3 corresponding to amino acids 1-392, 1-393, 1-394, 1-395 or 1-396 of dengue type 4 envelope glycoprotein (amino acids 392-396 are set forth in FIG. 9);
• and
• amino acids 1-398, 1-399, 1-400, 1-401 or 1-402 of Japanese encephalitis virus (amino acids 398-402 are set forth in FIG. 11).