Pharmaceutical composition and dietary supplement containing docosarexaenoic acid obtained from dinoflagellates
AssigneeMartek Biosciences Corporation
InventorsDavid J. Kyle, Sue E. Reeb, Valerie J. Sicotte
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Abstract
A pharmaceutical composition and a dietary supplement comprising a single cell oil containing at least 20% docosahexaenoic acid (DHA) in triglyceride form are presented. The single cell oil is extracted from biomass produced by cultivating heterotrophic microalgae of the class Dinophyceae in an aerobic fermentor with a nutrient solution having a limited nitrogen source.
Claims7
A pharmaceutical composition comprising a single cell oil containing at least 20% docosahexaenoic acid (DHA) in the triglyceride form, produced by a process comprising:
- a) cultivating a heterotrophic microalgae of the class Dinophyceae capable of producing a single cell oil containing at least 20% DHA in the triglyceride form in an aerobic fermentor containing a nutrient solution having a limiting nitrogen source, said cultivation being continued to achieve a cell density of at least 10 grams of biomass per liter of nutrient solution, the concentration of the nitrogen source in the nutrient solution being limited sufficiently to induce said microalgae to produce the single cell oil at a concentration of at least 2 grams per liter of nutrient solution;
- and
- b) extracting said single cell oil containing at least 20% DHA in the triglyceride form from the biomass.
A dietary supplement comprising a single cell edible oil containing at least 20% docosahexaenoic acid (DHA) in the triglyceride form, produced by a process comprising:
- a) cultivating a heterotrophic microalgae of the class Dinophyceae capable of producing said single cell edible oil containing at least 20% DHA in the triglyceride form, in an aerobic fermentor containing a nutrient solution having a limiting nitrogen source, said cultivation being continued to achieve a cell density of at least 10 grams of biomass per liter of nutrient solution, the concentration of the nitrogen source in the nutrient solution being limited sufficiently to induce said microalgae to produce the single cell edible oil at a concentration of at least 2 grams per liter of nutrient solution;
- and
- b) extracting said single cell edible oil containing at least 20% DHA in the triglyceride form from the biomass.
Dependencies are inferred from the claim text.