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AssigneeLotte Confectionery Co., Ltd.
InventorsJang Youn Choi, Jae Ho Lee, Kee Hyuh Choi, Ik Boo Kwon
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The particular strain of Klebsiella oxytoca No. 19-1 has been isolated from soil which produces a cyclomaltoglucanotransferase enzyme capable of converting starch to .alpha.-cyclodextrin in very high proportion, nearly close to 100 percent, rather than another types of cyclodextrins.
An isolated microorganism which produces a cyclodextrin glycosyltransferase when cultivated with starch, in the presence of a nitrogen source under aerobic conditions, said cyclodextrin glycosyltransferase converts starch to cyclodextrin which is at least 95% .alpha.-cyclodextrin;
A biologically pure culture of Klebsiella oxytoca which produces a cyclodextrin glycosyltransferase when cultivated with starch, in the presence of a nitrogen source under aerobic conditions, said cyclodextrin glycosyltransferase converts starch to cyclodextrin, which is at least 95% .alpha.-cyclodextrin, wherein said Klebsiella oxytoca is Klebsiella oxytoca No. 19-1 (KCCM 1002).
An isolated gram negative microorganism which hydrolyzes pectate, grows at 10.degree. C., is fecal coliform positive and produces cyclodextrin glycosyltransferase, when cultivated with starch, in the presence of a nitrogen source under aerobic conditions, said cyclodextrin glycosyltransferase converts starch to cyclodextrin which is at least 95% .alpha.-cyclodextrin, wherein said microorganism is Klebsiella oxytoca No. 19-1 (KCCM 1002).
A biologically pure culture of a gram negative microorganism which hydrolyzes pectate, grows at 10.degree. C., is fecal coliform positive and produces cyclodextrin glycosyltransferase when cultivated with starch, in the presence of a nitrogen source under aerobic conditions, said cyclodextrin glycosyltransferase converts starch to cyclodextrin which is at least 95% .alpha.-cyclodextrin wherein said microorganism is Klebsiella oxytoca No. 19-1 (KCCM 1002).
A biologically pure culture of Klebsiella oxytoca No. 19-1 (KCCM 1002).
Isolated Klebsiella oxytoca No. 19-1 (KCCM 1002).
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Bacteria; Culture media therefor: bacterial isolates
Preparation of compounds containing saccharide radicals: produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Bacteria or Actinomycetales ; using bacteria or Actinomycetales: klebsiella
Microorganisms: using bacteria or actinomycetales
Term ended 20 Feb 2013 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
The present invention relates to a newly isolated strain of Klebsiella oxytoca No. 19-1 which is capable of producing .alpha.-cyclodextrin in high proportion, nearly close to 100 percent, from starch.
Cyclodextrins (cycloamyloses, cyclomaltaoses, Schardinger dextrins, CDs) are cyclic, nonreducing oligossccharides composed of glucose units linked by .alpha.-1,4 glycosidic bonds. They are called .alpha.-, .beta.-, or .gamma.- cyclodextrin depending on the number of glucose units: 6, 7 or 8, respectively.
Cyclodextrins have been shown to act as host molecules, having a torus in the molecule capable of forming inclusion complexes with various kinds of organic compounds. This specific ability has been widely used in the field of food, pharmaceuticals, agricultural chemistry, cosmetics, and perfumes.
FIG. 1, the CGTase reaches its maximum activity after 9 hr cultivation and further increase of activity was not observed after that time.
FIG. 2A, the CGTase reveals that its optimum pH range is 5.5-7.5 and its maximum activity shows at around pH 6.0, and that no activity is found below pH 4.0 or above 10.0.
FIG. 2B shows the results of activity according to various temperature at pH 6.0. The CGTase is most active in the broad range of 30.degree.-50.degree. C., and the maximum activity of the CGTase was observed around 45.degree.-50.degree. C.
The particular strain of Klebsiella oxytoca No. 19-1 was isolated from soil which was collected from Kosung County, Kyungsangnam Province, Korea by the present inventors in 1990.
Taxonomical study of the strain was performed according to Bergey's Manual of Systematic Bacteriology and API 20E Kit(France).
Taxonomical characteristics of Klebsiella oxytoca No. 19-1 is as follows: