Apparatus and method for seeding endothelial cells

An apparatus for culturing living cells comprising:

• a hollow cylindrical cell growth container having two ends, each end being heat crimped, said cell growth container being operable to contain a cell culture, said cell growth container being operable to be rotated by a rotation means;
• a rotation container operable to contain said cell growth container:
• said rotation container being operable to be substantially fixedly attached in substantially synchronous relation with a rotation means;
• and
• retaining means positioned in contact with said cell growth container for enhancing a separated condition of said cell growth container within said rotation container, wherein said retaining means comprises at least one o-ring member.

An apparatus for culturing cells comprising:

• a hollow cylindrical synthetic graft having two ends, each end being heat crimped, said synthetic graft being operable to contain a cell culture, said synthetic graft being operable to be rotated by a rotation means;
• a rotation container operable to contain said synthetic graft, said rotation container being operable to be substantially fixedly attached in substantially synchronous relation with a rotation means;
• and
• a plurality of o-ring members positioned about said synthetic graft for enhancing a separated condition of said synthetic graft from said rotation container.

An apparatus for culturing cells comprising:

• a hollow cylindrical synthetic graft having two ends, each end being heat crimped, said synthetic graft being operable to contain a cell culture, said synthetic graft being operable to be rotated by a rotation means, and wherein said synthetic graft comprises an expanded-polytetrafiuoroethylene graft;
• a rotation container operable to contain said synthetic graft, said rotation container being operable to be substantially fixedly attached in substantially synchronous relation with a rotation means;
• and
• a plurality of o-ring members positioned about said synthetic graft for enhancing a separated condition of said synthetic graft from said rotation container.