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US 5,492,823 AGranted
Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli
AssigneeNew England Biolabs, Inc.
InventorShuang-yong Xu
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Abstract
The present invention discloses a novel method for the direct cloning of nuclease genes such as restriction endonuclease genes in E. coli. In addition, there is provided a novel strain which facilitates application of the method. This method has been successfully employed to clone a number of genes coding for endonuclease including restriction endonuclease genes.
Claims5
Isolated DNA coding for the BsoBl restriction endonuclease, wherein the isolated DNA is obtainable from the vector pBsoR1.
A recombinant DNA vector comprising a vector into which a DNA segment coding for the BsoBl restriction endonuclease has been inserted.
Dependencies are inferred from the claim text.