Stabilized polyacrylic saccharide protein conjugates

A water soluble protein polymer conjugate comprising a protein and an acrylic polymer having a main chain and a molecular weight of at least about 5,000, wherein three or more linker groups bond the protein to the acrylic polymer, the linker groups being covalently bonded to the protein and to the acrylic polymer, said linker groups having at least about three hydroxyl groups and not more than 60 carbon atoms, each of the linker groups being selected from the group consisting of ##STR25## wherein E=--OH or --SH;

• n=0 or 1;
• W=0 or 1;
• and
• Y=bond or --CH.sub.2 ;
• wherein A is bonded to the polymer and is selected from the group consisting of ##STR26## B is bonded to the protein and is selected from the group consisting of ##STR27## wherein R.sub.1 and R.sub.2 are each selected from the group consisting of H, OH, acetoxy, alkoxy, phenylene, straight chain alkyl or branched chain alkyl having 1 to 12 carbons, ##STR28## wherein x=2 to 7, ##STR29## 2-hydroxy ethyl, 3-chloro-2-hydroxypropyl, 2-hydroxy- 1-methylethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2, 3-dihydroxypropyl, 2 hydroxybutyl, 4-hydroxybutyl, diethylene-glycol, 5-hydroxypentryl, 6-hydroxyhexyl, triethyleneglycol, 7-hydroxyheptyl, 3, 4-dihydroxybutyl, 5-hydroxypentyl, 6-hydroxyhexyl, 2, 3-dimethyl-3- hydroxybutyl, 5,6-dihydroxyhexyl, 7-hydroxyheptyl, a residue of a mono-, di-, and trisaccharide;
• wherein R.sub.3 selected from the group consisting of H, straight chain alkyl or branched chain alkyl having 1 to 12 carbons, ##STR30## wherein x=2 to 7, ##STR31## 2-hydroxy ethyl, 3-chloro-2-hydroxypropyl, 2-hydroxy-1-methylethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2, 3-dihydroxypropyl, 2 hydroxybutyl, 4-hydroxybutyl, diethylene-glycol, 5-hydroxypentryl, 6-hydroxyhexyl, triethyleneglycol, 7-hydroxyheptyl, 3, 4-dihydroxybutyl, 5-hydroxypentyl, 6-hydroxyhexyl, 2, 3-dimethyl-3-hydroxybutyl, 5,6-dihydroxyhexyl, 7-hydroxyheptyl, a residue of a mono-, di-, and trisaccharide;
• and
• R'.sub.1,R'.sub.2, R'.sub.3, R'.sub.4, R'.sub.5, R'.sub.6, R'.sub.7, R'.sub.8, and R'.sub.9 each selected from the group consisting of H, OH, acetoxy, alkoxy, halogen and phosphate, straight chain alkyl or branched chain alkyl having 1 to 12 carbons, ##STR32## wherein x=2 to 7, ##STR33## 2-hydroxy ethyl, 3-chloro-2-hydroxypropyl, 2-hydroxy-1-methylethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2, 3-dihydroxypropyl, 2 hydroxybutyl, 4-hydroxybutyl, diethylene-glycol, 5-hydroxypentryl, 6-hydroxyhexyl, triethyleneglycol, 7-hydroxyheptyl, 3, 4-dihydroxybutyl, 5-hydroxypentyl, 6-hydroxyhexyl, 2, 3-dimethyl-3-hydroxybutyl, 5,6-dihydroxyhexyl, 7-hydroxyheptyl, residue of a mono-, di-, and trisaccharide.